Effect of astragaloside on cardiomyocyte apoptosis in murine coxsackievirus B3 myocarditis
Effect of astragaloside on cardiomyocyte apoptosis in murine coxsackievirus B3 myocarditis
复制标题
黄芪甲苷对小鼠柯萨奇病毒B3型心肌炎心肌细胞凋亡的影响
DOI：
10.1080/10286020412331286506
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发表时间：
2007-03-01
影响因子：
1.7
通讯作者：
Chen, H.-Z.
中科院分区：
文献类型：
--
作者：
Zhang, Z.-C.;Li, S.-J.;Chen, H.-Z.
黄芪甲苷是中草药黄芪的主要成分之一，对心血管系统具有正性肌力、抗心律失常、抗氧化等药理作用。采用末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法检测柯萨奇病毒B3(CVB3)感染的心肌炎小鼠心肌细胞凋亡及相关基因表达，观察黄芪甲苷对小鼠心肌细胞凋亡及相关基因表达的影响。结果表明，黄芪甲苷可抑制心肌细胞的凋亡及多种凋亡相关基因的表达。黄芪皂苷的抗细胞凋亡作用可能有助于改善黄芪制剂治疗心肌炎的临床疗效。
Astragaloside is the major component of Astragalus membranaceus, one of the Chinese medical herbs, and has several pharmacological actions on cardiovascular system, including positive inotropic, anti-arrhythmia and anti-oxidant activities. We have investigated the effect of astragaloside on cardiomyocyte apoptosis and expression of apoptosis-associated genes in mice with coxsackievirus B3 (CVB3)-induced myocarditis, the former of which has been detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay and the latter determined by using a focused microarray. Results showed that cardiomyocyte apoptosis and expression of various apoptosis associated genes are inhibited after treatment with astragaloside. The anti-apoptotic activity of astragaloside may contribute to the improvement of clinical outcomes in treating myocarditis with pharmaceutics of Astragalus membranaceus.