Solution Structure and Main Chain Dynamics of the Regulatory Domain (Residues 1–91) of Human Cardiac Troponin C*
Solution Structure and Main Chain Dynamics of the Regulatory Domain (Residues 1–91) of Human Cardiac Troponin C*
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人心肌肌钙蛋白 C* 调节域（残基 1-91）的溶液结构和主链动力学
DOI：
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发表时间：
1998
影响因子：
4.8
通讯作者：
T. Drakenberg
中科院分区：
文献类型：
--
作者：
Kimmo Pääkkönen;A. Annila;T. Sorsa;P. Pollesello;C. Tilgmann;I. Kilpeläinen;Piia Karisola;I. Ulmanen;T. Drakenberg
在水/三氟乙醇（91:9 v/v）溶液中，用核磁共振法测定了人心肌肌钙蛋白C （cNTnC）载钙调控结构域（n端）（1-91）的三维结构。单钙负载的心脏调节结构域呈“封闭”构象，相对较少暴露疏水表面。从Ca2+-cNTnC家族计算的差距矩阵，骨架TnC （sNTnC）结构的载脂蛋白和二钙形式显示出相似的N， A和D螺旋的相对取向。与2·Ca2+-sNTnC相比，Ca2+-cNTnC和apo-sNTnC中的B和C螺旋更接近NAD框架。然而，在不同温度下测量的几种氨基酸的宽15N共振表明存在构象交换。根据Lipari-Szabo模型，α-螺旋和钙结合环中的大多数酰胺表现出非常快的运动和相对较小的振幅。N端和C端有一些残基是挠性的。记录了未标记和15N标记样品的数据，并通过15N t1， t2和异核核Overhauser效应以及弛豫干涉测量研究了骨干动力学。
The three-dimensional structure of calcium-loaded regulatory, i.e. N-terminal, domain (1–91) of human cardiac troponin C (cNTnC) was determined by NMR in water/trifluoroethanol (91:9 v/v) solution. The single-calcium-loaded cardiac regulatory domain is in a “closed” conformation with comparatively little exposed hydrophobic surface. Difference distance matrices computed from the families of Ca2+-cNTnC, the apo and two-calcium forms of the skeletal TnC (sNTnC) structures reveal similar relative orientations for the N, A, and D helices. The B and C helices are closer to the NAD framework in Ca2+-cNTnC and in apo-sNTnC than in 2·Ca2+-sNTnC. However, there is an indication of a conformational exchange based on broad 15N resonances for several amino acids measured at several temperatures. A majority of the amides in the α-helices and in the calcium binding loop exhibit very fast motions with comparatively small amplitudes according to the Lipari-Szabo model. A few residues at the N and C termini are flexible. Data were recorded from nonlabeled and15N-labeled samples, and backbone dynamics was investigated by 15N T 1,T 2, and heteronuclear nuclear Overhauser effect as well as by relaxation interference measurements.
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DOI：
10.1016/s0021-9258(18)63868-2
发表时间：
1989-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Putkey;H L Sweeney;S T Campbell
通讯作者：
J. Putkey;H L Sweeney;S T Campbell
DOI：
10.1016/s0021-9258(19)70187-2
发表时间：
1980-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Holroyde;S. P. Robertson;J. Johnson;R. Solaro;J. D. Potter
通讯作者：
M. Holroyde;S. P. Robertson;J. Johnson;R. Solaro;J. D. Potter
影响因子：
2.9
作者：
Spyracopoulos, L;Li, MX;Sykes, BD
通讯作者：
Sykes, BD
鸡骨骼肌肌钙蛋白 C 在二钙状态下的精细结构，分辨率为 2-A。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Satyshur,KA;Rao,ST;Pyzalska,D;Drendel,W;Greaser,M;Sundaralingam,M
通讯作者：
Sundaralingam,M