Identification, Quantification, and Anti-inflammatory Activity of 5-n-Alkylresorcinols from 21 Different Wheat Varieties
Identification, Quantification, and Anti-inflammatory Activity of 5-n-Alkylresorcinols from 21 Different Wheat Varieties
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21 个不同小麦品种中 5-n-烷基间苯二酚的鉴定、定量和抗炎活性
DOI：
10.1021/acs.jafc.8b02911
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发表时间：
2018-09-05
影响因子：
6.1
通讯作者：
Wang, Jing
中科院分区：
文献类型：
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作者：
Liu, Jie;Hao, Yiming;Wang, Jing
研究了21份中国小麦麸皮样品中5-n-烷基间苯二酚（ARs）的特征成分及其抗炎活性。样品中ARs的含量在697 ~ 1732 μ g/g之间，由5种不同的同源物组成。在这些同源物中，C19:0和C21:0最为丰富，其次是C17:0、C23:0和C25:0。此外，补充ARs可显著抑制lps激活的RAW264.7巨噬细胞中IL-1 β、IL-6和tnf - α的mRNA表达。其抗炎作用的分子机制可能与抑制核因子κ B （nf - κ B）和JNK/MAPK的激活有关。ARs处理显著降低NF-kappa B p65核易位和抑制剂kappa B （I kappa B α）激酶和JNK磷酸化。此外，ARs同源物C17:0已被证明是主要的活性成分。本研究结果可为促进小麦及其副产品的综合利用，改善人类健康提供参考。
The characteristic constituent and anti-inflammatory activity of 5-n-alkylresorcinols (ARs) from 21 wheat bran samples in China were investigated in this study. The amount of ARs ranged from 697 to 1732 mu g/g in the tested samples, which were composed of five different homologues. Among these homologues, C19:0 and C21:0 were the most abundant, followed by C17:0, C23:0, and C25:0. Moreover, the mRNA expression of IL-1 beta, IL-6, and TNF-alpha in LPS-activated RAW264.7 macrophage cells were significantly inhibited by ARs supplementation. The molecular mechanisms behind its anti-inflammatory activity could result from the suppression of nuclear factor-kappa B (NF-kappa B) and JNK/MAPK activation. ARs treatment notably decreased NF-kappa B p65 nuclear translocation and inhibitor kappa B (I kappa B alpha) kinase and JNK phosphorylation. Additionally, ARs homologues C17:0 had been proven to be the main active constituent. The results from this study could be used to promote the comprehensive utilization of wheat and its byproducts in improving human health.