PRSS37 deficiency leads to impaired energy metabolism in testis and sperm revealed by DIA-based quantitative proteomic analysis
PRSS37 deficiency leads to impaired energy metabolism in testis and sperm revealed by DIA-based quantitative proteomic analysis
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基于 DIA 的定量蛋白质组学分析显示，PRSS37 缺陷会导致睾丸和精子的能量代谢受损
DOI：
10.1007/s43032-022-00918-x
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发表时间：
2022-04
影响因子：
2.9
通讯作者：
Zhugang Wang
中科院分区：
文献类型：
--
作者：
Wenfeng Xiong;Haoyang Ge;Chunling Shen;Chaojie Li;Xiaohong Zhang;Lingyun Tang;Yan Shen;Shunyuan Lu;Hongxin Zhang;Zhugang Wang
我们先前的研究报道，一种假定的类胰蛋白酶丝氨酸蛋白酶PRSS37在精子发生后期仅在睾丸生殖细胞中表达，并且对精子从子宫迁移到输卵管以及精卵识别至关重要。
Our previous studies have reported that a putative trypsin-like serine protease, PRSS37, is exclusively expressed in testicular germ cells during late spermatogenesis and essential for sperm migration from the uterus into the oviduct and sperm-egg recognition via mediating the interaction between PDILT and ADAM3. In the present study, the global proteome profiles of wild-type (wt) andPrss37−/−mice in testis and sperm were compared employing data independent acquisition (DIA) technology. Overall, 2506 and 459 differentially expressed proteins (DEPs) were identified inPrss37-null testis and sperm, respectively, when compared to control groups. Bioinformatic analyses revealed that most of DEPs were related to energy metabolism. Of note, the DEPs associated with pathways for the catabolism such as glucose via glycolysis, fatty acids via β-oxidation, and amino acids via oxidative deamination were significantly down-regulated. Meanwhile, the DEPs involved in the tricarboxylic acid cycle (TCA cycle) and oxidative phosphorylation (OXPHOS) were remarkably decreased. The DIA data were further confirmed by a markedly reduction of intermediate metabolites (citrate and fumarate) in TCA cycle and terminal metabolite (ATP) in OXPHOS system after disruption of PRSS37. These outcomes not only provide a more comprehensive understanding of the male fertility of energy metabolism modulated by PRSS37 but also furnish a dynamic proteomic resource for further reproductive biology studies.
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DOI：
10.1002/9780470015902.a0003067.pub2
发表时间：
2001-04
期刊：
--
影响因子：
--
作者：
Padmini P. Polinati;Petra M Eskelin;T. Tyni
通讯作者：
Padmini P. Polinati;Petra M Eskelin;T. Tyni
影响因子：
6.6
作者：
Izraely S;Ben-Menachem S;Sagi-Assif O;Meshel T;Malka S;Telerman A;Bustos MA;Ramos RI;Pasmanik-Chor M;Hoon DSB;Witz IP
通讯作者：
Witz IP
影响因子：
6.4
作者：
Signes A;Fernandez-Vizarra E
通讯作者：
Fernandez-Vizarra E
DOI：
10.1016/s0006-291x(05)81033-2
发表时间：
1990-11
影响因子：
3.1
作者：
E. Nanba;K. Suzuki
通讯作者：
E. Nanba;K. Suzuki
影响因子：
4.2
作者：
van de Ven, Steffi;Gardeitchik, Thatjana;Morava, Eva
通讯作者：
Morava, Eva