ESPVR of in situ rat left ventricle shows contractility-dependent curvilinearity
ESPVR of in situ rat left ventricle shows contractility-dependent curvilinearity
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DOI：
10.1152/ajpheart.1998.274.5.h1429
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发表时间：
1998-05-01
影响因子：
4.8
通讯作者：
Sunagawa, K
中科院分区：
文献类型：
--
作者：
Sato, T;Shishido, T;Sunagawa, K
我们开发了一种用于原位大鼠左心室 (LV) 容量测定的小型化电导导管。在对 11 只大鼠进行电导容量法验证研究后，我们对 24 只去主动脉神经、迷走神经切断和乌拉坦麻醉的大鼠进行了收缩末期压力-容量关系 (SPIVERY) 的表征。使用电导导管测量的每搏输出量 (SV) 与电磁流量计测量的结果密切相关 (r > 0.95)。通过电导容量法和死后形态测量法测量的原位左心室舒张末期容积之间没有发现显着差异；线性回归分析表明，相关系数为0.934，斜率与1没有显着差异，截距与0没有显着差异。在心脏交感紧张状态下，ESPVR呈曲线形。在收缩末期压力为 100 mmHg 时通过二次曲线拟合得出的 ESPVR（收缩末弹性，E-es）的估计斜率为 2,647 +/- 846 mmHg/ml。双侧颈神经节和星状神经节切除术降低了收缩力并使 ESPVR 呈线性；二次方程并没有改善拟合。 E-es 为 946 +/- 55 mmHg/ml，体积轴 (V-0) 截距为 0.076 +/- 0.007 ml。普萘洛尔 (1 mg/kg) 的给药进一步降低了 E-es (573 +/- 61 mmHg/ml, P < 0.001)，并略微增加了 V-0 (0.091 +/- 0.011 ml)。我们得出的结论是，电导导管方法可用于评估原位大鼠左心室的 ESPVR，并且 ESPVR 显示出收缩性依赖性曲线。
We developed a miniaturized conductance catheter for in situ rat left ventricular (LV) volumetry. After the validation study of the conductance volumetry in 11 rats, we characterized the end-systolic pressure-volume relationship (SPIVERY) in 24 sinoaortic-denervated, vagotomized and urethan-anesthetized rats. Stroke volume (SV) measured with the conductance catheter correlated closely with that measured by electromagnetic flowmetry (r > 0.95). No significant difference was found between the in situ LV end-diastolic volumes measured by conductance volumetry and postmortem morphometry; a linear regression analysis indicated that the correlation coefficient was 0.934, that the slope was not significantly different from 1, and that the intercept was not significantly different from 0. During cardiac sympathotonic conditions, the ESPVR was curvilinear. The estimated slope of ESPVR (end-systolic elastance, E-es) by quadratic curve fitting at end-systolic pressure of 100 mmHg was 2,647 +/- 846 mmHg/ml. Bilateral cervical and stellate ganglionectomy depressed contractility and made the ESPVR linear; a quadratic equation did not improve the fit. E-es was 946 +/- 55 mmHg/ml with the volume-axis (V-0) intercept of 0.076 +/- 0.007 ml. Administration of propranolol (1 mg/kg) further reduced E-es (573 +/- 61 mmHg/ml, P < 0.001) and increased V-0 slightly (0.091 +/- 0.011 ml). We conclude that the conductance catheter method is useful for the assessment of the ESPVR of the in situ rat left ventricle and that the ESPVR displays contractility-dependent curvilinearity.