Chemical Composition, Anti-Breast Cancer Activity and Extraction Techniques of Ent-Abietane Diterpenoids from Euphorbia fischeriana Steud.
Chemical Composition, Anti-Breast Cancer Activity and Extraction Techniques of Ent-Abietane Diterpenoids from Euphorbia fischeriana Steud.
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大戟中对映松香烷二萜的化学成分、抗乳腺癌活性及提取工艺
DOI：
10.3390/molecules27134282
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发表时间：
2022-07-03
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影响因子：
--
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中科院分区：
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--
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对映松香烷型二萜是狼毒大戟的主要活性成分。为寻找新的抗乳腺癌药物，从E. Fischeriana。根据1D和2D NMR谱以及HRESIMS数据，这些化合物的结构被清楚地阐明。其中化合物1为新化合物，化合物10为首次从大戟属植物中分离得到，化合物11为首次从大戟属植物中发现。Fischeriana。这些化合物在体外对MCF-10A、MCF-7、ZR-75-1和MDA-MB-231细胞系表现出不同程度的生长抑制。所获得的实验数据使我们能够确定环氧基，羟基和乙酰氧基对它们的细胞毒活性的作用。提取是从天然植物中分离、鉴定和应用有价值化合物的重要手段。为了提高E.以Fischeriana、17-羟基焦木黄酮B、焦木黄酮B、17-羟基焦木黄酮A和焦木黄酮A为对照品，采用响应面法优化盐析辅助液液萃取工艺。SALLE的优化条件为0.47 g磷酸二氢钠、5.5 mL乙腈和4.5 mL水（pH 7.5）。17-羟基jolkinase B、jolkinase B、17-羟基jolkinase A和jolkinase A的实验值（分别为2.134、0.529、0.396和0.148 mg/g）与预测值一致，从而证明了模型的适当性。
Ent-abietane diterpenoids are the main active constituents of Euphorbia fischeriana. In the continuing search for new anti-breast cancer drugs, 11 ent-abietane diterpenoids (1–11) were isolated from E. fischeriana. The structures of these compounds were clearly elucidated on the basis of 1D and 2D NMR spectra as well as HRESIMS data. Among them, compound 1 was a novel compound, compound 10 was isolated from Euphorbia genus for the first time, compound 11 was firstly discovered from E. fischeriana. These compounds exhibited varying degrees of growth inhibition against the MCF-10A, MCF-7, ZR-75-1 and MDA-MB-231 cell lines in vitro. The experimental data obtained permit us to identify the roles of the epoxy group, hydroxyl group and acetoxyl group on their cytotoxic activities. Extraction is an important means for the isolation, identification, and application of valuable compounds from natural plants. To maximize yields of ent-abietane diterpenoids of E. fischeriana, 17-hydroxyjolkinolide B, jolkinolide B, 17-hydroxyjolkinolide A and jolkinolide A were selected as quality controls to optimize the salting-out-assisted liquid–liquid extraction (SALLE) by response surface methodology (RSM). The optimized conditions for SALLE were 0.47 g sodium dihydrogen phosphate, 5.5 mL acetonitrile and 4.5 mL water at pH 7.5. The experimental values of 17-hydroxyjolkinolide B, jolkinolide B, 17-hydroxyjolkinolide A and jolkinolide A (2.134, 0.529, 0.396, and 0.148 mg/g, respectively) were in agreement with the predicted values, thus demonstrating the appropriateness of the model.
E. fischeriana 根化合物 Dpo 激活抗病毒先天免疫
影响因子：
5.7
作者：
Chen J;Du H;Cui S;Liu T;Yang G;Sun H;Tao W;Jiang B;Yu L;You F
通讯作者：
You F