Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge.
Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge.
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DOI：
10.1371/journal.pone.0026041
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ye L
中科院分区：
文献类型：
--
作者：
Zhang T;Zhang XX;Ye L
抗生素的过度使用或误用加速了抗生素耐药性的产生，给世界公共卫生带来了重大挑战。污水处理厂(STP)是抗生素耐药基因(ARGs)的重要储存库，活性污泥具有高微生物密度和多样性的特点，可通过可移动遗传元件(MGES)促进ARG水平基因转移(HGT)。然而，关于污泥微生物群中的ARGs和MGES池的情况知之甚少。本研究利用转座子辅助捕获(Traca)系统从香港一株STP中国的活性污泥中分离出新的质粒。我们还利用Illumina HiSeq 2000高通量测序和元基因组学分析对该质粒元基因组进行了研究。利用TRACA系统从污泥微生物组中获得了两个新的质粒，通过元基因组学分析鉴定了一个新的质粒。我们的结果揭示了HGT的各种ARGs和MGES的高水平，包括整合子、转座子和质粒。应用TRACA系统从环境元基因组中分离新的质粒，再加上随后的高通量测序和元基因组分析，突显了ARGs和MGES在STP微生物群落中的普遍存在。
The overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elements (MGEs). However, little is known regarding the pool of ARGs and MGEs in sludge microbiome. In this study, the transposon aided capture (TRACA) system was employed to isolate novel plasmids from activated sludge of one STP in Hong Kong, China. We also used Illumina Hiseq 2000 high-throughput sequencing and metagenomics analysis to investigate the plasmid metagenome. Two novel plasmids were acquired from the sludge microbiome by using TRACA system and one novel plasmid was identified through metagenomics analysis. Our results revealed high levels of various ARGs as well as MGEs for HGT, including integrons, transposons and plasmids. The application of the TRACA system to isolate novel plasmids from the environmental metagenome, coupled with subsequent high-throughput sequencing and metagenomic analysis, highlighted the prevalence of ARGs and MGEs in microbial community of STPs.