Assessment of DNA Encapsulation, a New Room-Temperature DNA Storage Method
Assessment of DNA Encapsulation, a New Room-Temperature DNA Storage Method
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DOI：
10.1089/bio.2013.0082
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发表时间：
2014-06-01
影响因子：
1.6
通讯作者：
Bizet, Chantal
中科院分区：
文献类型：
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作者：
Clermont, Dominique;Santoni, Sylvain;Bizet, Chantal
对一种新的DNA室温储存方法进行了评估，其中将来自人体组织、细菌和植物的DNA样品储存在装有不锈钢激光密封胶囊（DNAshells（R））的小玻璃小瓶中，置于缺氧和无水气氛下。将样品在室温下储存在DNAshells中不同的时间段，以评估任何降解，并将其与冷冻对照样品和储存在GenTegraTM管中的样品进行比较。该研究包括使用高温（76 ℃）和50%相对湿度对加速老化的影响进行分析。在室温下储存在DNAshells（R）中18个月的样品中没有观察到可检测的DNA降解。聚合酶链反应实验、脉冲场凝胶电泳和扩增片段长度多态性分析也表明，DNA壳（R）的保护性能不受极端条件（76摄氏度，50%湿度）下储存30小时的影响，保证100年不发生DNA样品降解。然而，在76摄氏度下储存30小时后，有必要对过程进行调整，以避免DNA损失。通过加入海藻糖，可提供保护基质，在高温下储存1周甚至1个月，获得DNA的成功保护。这项研究证明了使用DNAshells（R）进行室温储存的许多优点，特别是在长期稳定性，安全性，运输和分子生物学研究应用方面。
A new procedure for room-temperature storage of DNA was evaluated whereby DNA samples from human tissue, bacteria, and plants were stored under an anoxic and anhydrous atmosphere in small glass vials fitted in stainless-steel, laser-sealed capsules (DNAshells (R)). Samples were stored in DNAshells (R) at room temperature for various periods of time to assess any degradation and compare it to frozen control samples and those stored in GenTegra (TM) tubes. The study included analysis of the effect of accelerated aging by using a high temperature (76 degrees C) at 50% relative humidity. No detectable DNA degradation was seen in samples stored in DNAshells (R) at room temperature for 18 months. Polymerase chain reaction experiments, pulsed field gel electrophoresis, and amplified fragment length polymorphism analyses also demonstrated that the protective properties of DNAshells (R) are not affected by storage under extreme conditions (76 degrees C, 50% humidity) for 30 hours, guaranteeing 100 years without DNA sample degradation. However, after 30 hours of storage at 76 degrees C, it was necessary to include adjustments to the process in order to avoid DNA loss. Successful protection of DNA was obtained for 1 week and even 1 month of storage at high temperature by adding trehalose, which provides a protective matrix. This study demonstrates the many advantages of using DNAshells (R) for room-temperature storage, particularly in terms of long-term stability, safety, transport, and applications for molecular biology research.