Projections of the lateral reticular nucleus to the cochlear nucleus in rats.
Projections of the lateral reticular nucleus to the cochlear nucleus in rats.
复制标题
大鼠侧网状核到耳蜗核的投射。
DOI：
10.1002/cne.21463
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Ryugo,DavidK
中科院分区：
文献类型：
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作者：
Zhan,Xiping;Ryugo,DavidK
外侧网状核（LRN）位于延髓吻侧和脑桥尾侧，参与心血管调节和颅神经反射，并在小脑中产生苔藓纤维。逆行追踪数据显示，中等大小的多极细胞从LRN的大细胞部分到耳蜗核（CN）。我们试图用BDA注射表征LRN向CN的投射。顺行标记的同侧CN末端显示钮扣和苔状纤维，光镜和电镜检查。CN的终末场局限于颗粒细胞结构域（GCD），特别是沿前腹侧CN的浅层和颗粒细胞层。电镜观察显示，最小的LRN钮扣形成1-3个突触，随着钮扣尺寸的增大，相应形成的突触也更多。最大的钮扣与最小的苔藓纤维无法区分，最大的苔藓纤维有15个突触。突触与圆形囊泡不对称，形成于薄的树突轮廓上，其特征是有大量的微管和细的丝状延伸穿过末端。这些突触后靶点的结构特征是颗粒细胞末端树突爪的特征。LRN预测与已知的非听觉输入GCD的组织原则一致。[j]中华神经医学杂志，2004,25(4):583 - 598。©2007 Wiley‐Liss, Inc。
The lateral reticular nucleus (LRN) resides in the rostral medulla and caudal pons, is implicated in cardiovascular regulation and cranial nerve reflexes, and gives rise to mossy fibers in the cerebellum. Retrograde tracing data revealed that medium‐sized multipolar cells from the magnocellular part of the LRN project to the cochlear nucleus (CN). We sought to characterize the LRN projection to the CN using BDA injections. Anterogradely labeled terminals in the ipsilateral CN appeared as boutons and mossy fibers, and were examined with light and electron microscopy. The terminal field in the CN was restricted to the granule cell domain (GCD), specifically in the superficial layer along the anteroventral CN and in the granule cell lamina. Electron microscopy showed that the smallest LRN boutons formed 1–3 synapses, and as boutons increased in size, they formed correspondingly more synapses. The largest boutons were indistinguishable from the smallest mossy fibers, and the largest mossy fiber exhibited 15 synapses. Synapses were asymmetric with round vesicles and formed against thin dendritic profiles characterized by plentiful microtubules and the presence of fine filopodial extensions that penetrated the ending. These structural features of the postsynaptic target are characteristic of the terminal dendritic claw of granule cells. LRN projections are consistent with known organizational principles of non‐auditory inputs to the GCD. J. Comp. Neurol. 504:583–598, 2007. © 2007 Wiley‐Liss, Inc.