Increased sequence coverage of thymine-rich oligodeoxynucleotides by infrared multiphoton dissociation compared to collision-induced dissociation.
Increased sequence coverage of thymine-rich oligodeoxynucleotides by infrared multiphoton dissociation compared to collision-induced dissociation.
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与碰撞诱导解离相比，红外多光子解离增加了富含胸腺嘧啶的寡脱氧核苷酸的序列覆盖率。
DOI：
10.1002/jms.1780
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Brodbelt,JenniferS
中科院分区：
文献类型：
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作者：
Parr,Carol;Brodbelt,JenniferS
在线性离子阱质谱仪中，富含胸腺嘧啶的寡脱氧核苷酸的红外多光子解离（IRMPD）比传统的碰撞诱导解离（CID）提供更广泛的裂解。对于含有一个非胸腺嘧啶碱基的寡脱氧核苷酸，CID主要导致非胸腺嘧啶核碱基的3′侧裂解，而IRMPD导致所有核碱基之间的裂解，从而提供完整的序列覆盖。此外，对于含有单个非胸腺嘧啶碱基的寡脱氧核苷酸，研究表明，IRMPD质谱中观察到的全系列诊断序列离子是由位于非胸腺嘧啶碱基旁边的初始切割位点形成的两种主要产物的二次解离产生的。版权所有© 2010约翰威利父子有限公司.
Infrared multiphoton dissociation (IRMPD) of thymine‐rich oligodeoxynucleotides in a linear ion‐trap mass spectrometer affords far more extensive fragmentation than conventional collision‐induced dissociation (CID). For oligodeoxynucleotides containing one non‐thymine base, CID results primarily in cleavage on the 3′ side of the non‐thymine nucleobase, whereas IRMPD results in cleavages between all the nucleobases and thus provides complete sequence coverage. Furthermore, for oligodeoxynucleotides containing a single non‐thymine base, it is shown that the full series of diagnostic sequence ions observed in the IRMPD mass spectra arise from secondary dissociation of the two primary products formed from the initial cleavage site located next to the non‐thymine base. Copyright © 2010 John Wiley & Sons, Ltd.