INACTIVATED INFLUENZA VACCINE EFFICACY - DIMINISHED ANTIGENICITY OF SPLIT-PRODUCT VACCINES IN MICE
INACTIVATED INFLUENZA VACCINE EFFICACY - DIMINISHED ANTIGENICITY OF SPLIT-PRODUCT VACCINES IN MICE
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DOI：
10.1128/iai.10.6.1329-1336.1974
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发表时间：
1974-01-01
影响因子：
3.1
通讯作者：
MAYNER, RE
中科院分区：
文献类型：
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作者：
BARRY, DW;STATON, E;MAYNER, RE
每组 60 至 120 只小鼠腹腔内接种不同稀释度的商业制备和许可的二价（A/英格兰和 B/马萨诸塞州）和单价（A/爱知或 B/香港）灭活流感疫苗，并通过血凝抑制测试对 14 天时的抗体反应进行定量。用 Tween-80 和磷酸三丁酯或乙醚处理 A/英格兰、B/马萨诸塞州和 B/香港全病毒制备的分割产品疫苗产生的平均抗体滴度显着低于同等全病毒制剂。当给予这些分装疫苗时，不同测试稀释度的血清转化率（<1:8 至≥1:8）也显着降低。当通过鸡细胞凝集试验对所有疫苗的抗原含量进行定量时，需要比全病毒抗原多 10 至 100 倍的裂解产物抗原才能在 50% 的测试小鼠中产生血清转化。分割产品和全病毒 A/Aichi 疫苗之间的差异不太明显。这些数据指出，在确定灭活流感疫苗的免疫原性时，需要考虑除血凝素含量以外的其他因素。
Groups of 60 to 120 mice were given a single intraperitoneal inoculation of varying dilutions of commercially prepared and licensed bivalent (A/England and B/Mass) and monovalent (A/Aichi or B/Hong Kong) inactivated influenza vaccines, and their antibody responses at 14 days were quantitated by hemagglutination inhibition tests. Split-product vaccines prepared by the treatment of A/England, B/Mass, and B/Hong Kong whole virus with Tween-80 and either tributylphosphate or ether produced significantly lower mean antibody titers than did equivalent whole-virus preparations. The rates of seroconversion (<1:8 to ≥1:8) at the various dilutions tested were also significantly reduced when these split-product vaccines were given. When the antigen content of all vaccines was quantitated by the chick cell agglutination test, between 10 and 100 times more split-product antigen than whole-virus antigen was required to produce seroconversion in 50% of the mice tested. Differences between split-product and whole-virus A/Aichi vaccines were less marked. These data point out the need to consider factors other than hemagglutinin content alone in determining the immunogenicity of inactivated influenza vaccines.