Regulation of the Bacillus subtilis extracytoplasmic function protein σΥ and its target promoters
Regulation of the Bacillus subtilis extracytoplasmic function protein σΥ and its target promoters
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DOI：
10.1128/jb.185.16.4883-4890.2003
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发表时间：
2003-08-01
影响因子：
3.2
通讯作者：
Helmann, JD
中科院分区：
文献类型：
--
作者：
Cao, M;Salzberg, L;Helmann, JD
枯草芽孢杆菌细胞质外功能sigma因子sigma（Y）的功能未知。我们证明了sigY操纵子是从一个自调节启动子位点P-Y表达的。我们选择了上调P-Y转录的转座子诱导的突变，试图鉴定参与sigma（Y）调节的基因。由此产生的插入破坏了sigY的下游基因yxlC。然而，yxlC：：TnlO插入的表型是由于sigY操纵子的下游基因上的极性; yxlC中的非极性插入不导致P-Y的去阻遏。进一步的分析显示，yxlD和yxlE编码的蛋白质对于σ（Y）活性的负调节是重要的。野生型和yxlC：：Tn 10突变株的转录组的比较揭示了几个操纵子的表达升高。然而，只有一个额外的基因ybgB被明确鉴定为sigma（Y）的直接靶标。这得到了用全基因组径流转录分析sigma（Y）转录的直接靶标，然后进行宏阵列分析的支持。
The Bacillus subtilis extracytoplasmic function sigma factor sigma(Y) is of unknown function. We demonstrate that the sigY operon is expressed from an autoregulatory promoter site, P-Y. We selected for transposon-induced mutations that upregulate P-Y transcription in an attempt to identify genes involved in sigma(Y) regulation. The resulting insertions disrupted yxlC, the gene immediately downstream of sigY. However, the phenotype of the yxlC::Tn10 insertion was due to polarity on the downstream genes of the sigY operon; a nonpolar insertion in yxlC did not lead to derepression of P-Y. Further analyses revealed that both yxlD and yxlE encoded proteins important for the negative regulation of sigma(Y) activity. A comparison of the transcriptomes of wild-type and yxlC:: Tn10 mutant strains revealed elevated expression of several operons. However, only one additional gene, ybgB, was unambiguously identified as a direct target for sigma(Y). This was supported by analysis of direct targets for sigma(Y) transcription with whole-genome runoff transcription followed by macroarray analysis.