The nucleotide sequence of Saccharomyces cerevisiae chromosome IX
The nucleotide sequence of Saccharomyces cerevisiae chromosome IX
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DOI：
10.1038/387s084
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发表时间：
1997-05-29
期刊：
影响因子：
64.8
通讯作者：
Barrell, B
中科院分区：
文献类型：
--
作者：
Churcher, C;Bowman, S;Barrell, B
大规模系统测序通常依赖于所研究的生物体的大插入细菌或病毒基因组克隆的有序文库的可用性。这些大型插入文库的产生以及每个克隆在基因组图谱上的定位是一个费力且耗时的过程。为了克服这些问题，几个研究小组已经成功地证明了全基因组随机“鸟枪法”在病毒和原核生物的大规模测序中的可行性（1-5）。在这里，我们报告的酿酒酵母染色体IX的序列，确定部分由一个完整的染色体“猎枪”，并描述在随机“猎枪”测序的整个真核染色体遇到的特殊困难。对该序列的分析表明，染色体IX含有221个开放阅读框（ORF），其中约30%先前已被测序。这条染色体显示出典型的小酿酒酵母染色体的特征。
Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-consuming process. In an effort to overcome these problems, several groups have successfully demonstrated the viability of the whole-genome random 'shotgun' method in large-scale sequencing of both viruses and prokaryotes(1-5). Here we report the sequence of Saccharomyces cerevisiae chromosome IX, determined in part by a whole-chromosome 'shotgun', and describe the particular difficulties encountered in the random 'shotgun' sequencing of an entire eukaryotic chromosome. Analysis of this sequence shows that chromosome IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome.