Interfacial self-assembly of fungal hydrophobins of the lichen-forming ascomycetes Xanthoria parietina and X-ectaneoides
Interfacial self-assembly of fungal hydrophobins of the lichen-forming ascomycetes Xanthoria parietina and X-ectaneoides
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DOI：
10.1006/fgbi.2000.1205
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发表时间：
2000-06-01
影响因子：
3
通讯作者：
Honegger, R
中科院分区：
文献类型：
--
作者：
Scherrer, S;De Vries, OMH;Honegger, R
在形成地衣的子囊菌Xanthoria parietina和X.ectaneoids的共生表型中，粘性、亲水性的皮层包裹着具有疏水壁面的气生菌丝系统，在X中，Parietina冷冻断裂电子显微镜显示，真菌和藻类的壁面上覆盖着一层棒状层。从这两个物种分离的热的十二烷基硫酸钠不溶于壁的残留物的提取物中含有一种蛋白质，该蛋白质在界面自组装上显示了一层棒状物。用RT-PCR方法克隆了XEH1(XH1，ectaneoides)和XPH1(XH1，parietina)的基因片段，对地衣菌种和非共生共生菌的基因组DNA印迹分析表明，XPH1和XEH1是真菌的单拷贝基因。两个编码蛋白的氨基酸序列同源性为96%，显示出疏水蛋白类的特征。(C)2000年学术出版社。
In the symbiotic phenotype of the lichen-forming ascomycetes Xanthoria parietina and X. ectaneoides, a conglutinate, hydrophilic cortex surrounds a system of aerial hyphae with hydrophobic wall surfaces, In X, parietina freeze-fracture electron microscopy showed that a rodlet layer covers the fungal and algal wall surfaces. Extracts of hot SDS-insoluble wall residues isolated from both species contained a protein that revealed a rodlet layer upon interfacial self-assembly. The N-terminal sequence of the 10-kDa protein of X, ectaneoides served to clone cDNA fragments of XEH1 (H1 of X, ectaneoides) and XPH1 (H1 of X, parietina) by RT-PCR, Genomic DNA blot analysis with both lichenized species and the aposymbiotically cultured symbionts of X, parietina showed that XPH1 and XEH1 ave fungal single copy genes. The deduced amino acid sequences of the two encoded proteins were 96% identical and showed the characteristics of class hydrophobins. (C) 2000 Academic Press.