Red-Shifted Environmental Fluorophores and Their Use for the Detection of Gram-Negative Bacteria
Red-Shifted Environmental Fluorophores and Their Use for the Detection of Gram-Negative Bacteria
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DOI：
10.3390/chemosensors9060117
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发表时间：
2021-05
期刊：
影响因子：
4.2
通讯作者：
A. Megia-Fernandez;Maxime Klausen;B. Mills;Gillian E. Brown;H. McEwan;N. Finlayson;K. Dhaliwal;M. Bradley
中科院分区：
文献类型：
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作者：
A. Megia-Fernandez;Maxime Klausen;B. Mills;Gillian E. Brown;H. McEwan;N. Finlayson;K. Dhaliwal;M. Bradley
利用微波辅助合成方法，合成了两种新型的水溶性花菁荧光团。在一系列质子型和非质子型溶剂中进行了全光学表征，染料在红色区域显示出荧光，在水中的亮度降低了20倍，表现出强烈的环境敏感性，在此称为生溶剂-荧光性(区别于溶剂变色性)。在水中也测量到了较短的荧光寿命，这证实了这一特征。这些染料被连接到一个改性的多粘菌素支架上，该支架允许在结合到革兰氏阴性细菌膜上时启动荧光，并在免洗的程序中选择性地检测细菌。
Two novel, water-soluble, merocyanine fluorophores were readily prepared by microwave-assisted synthesis. Full optical characterization was performed in a series of protic and aprotic solvents, and the dyes displayed fluorescence in the red region with up to a 20-fold decrease in brightness in water, demonstrating a strong environmental sensitivity hereby termed as solvato-fluorogenicity (to distinguish from solvatochromism). Shorter fluorescent lifetimes were also measured in water, which confirmed this character. These dyes were conjugated to a modified polymyxin scaffold that allowed fluorescence “switch-on” upon binding to Gram-negative bacterial membranes, and selective fluorescence detection of bacteria in a wash-free protocol.