Distribution of neuropeptide W immunoreactivity and mRNA in adult rat brain
Distribution of neuropeptide W immunoreactivity and mRNA in adult rat brain
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DOI：
10.1016/j.brainres.2006.03.041
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发表时间：
2006-06-06
期刊：
影响因子：
2.9
通讯作者：
Sakurai, Takeshi
中科院分区：
文献类型：
--
作者：
Kitamura, Yoji;Tanaka, Hirokazu;Sakurai, Takeshi
神经肽W（NPW）是最近发现的一种与G蛋白偶联受体（GPR）7结合的神经肽，GPR 7在啮齿类动物脑的几个离散区域（包括中央杏仁核和终纹床核）中高度表达。虽然一些报告表明，NPW参与能量稳态和伤害性感受的调节，但NPW的确切生理作用仍不清楚。在这项研究中，我们研究了NPW信使RNA和NPW免疫反应性在成年大鼠脑中的分布。NPW免疫反应（IR）细胞在腹侧被盖区，中脑导水管周围灰质，Edinger-Westphal核。NPW-ir纤维分布于外侧隔、纹状体床核等脑区。终末核、下丘脑背内侧核和后丘脑、中央杏仁核、海马CA 1区、脚间核、下丘、臂旁外侧核、面神经核和舌下神经核。NPW-IR纤维在杏仁中央核和纹状体床核中最丰富。终末肌，这是与恐惧和焦虑有关的区域。这些结果表明，NPW可能参与了应激和情绪反应的调节，特别是与恐惧和焦虑相关的生理和行为功能。(c)2006 Elsevier B. V.保留所有权利。
Neuropeptide W (NPW) is a recently identified neuropeptide that binds to G-protein-coupled receptor (GPR) 7, which is highly expressed in several discrete regions of the rodent brain including the central amygdaloid nucleus and bed nucleus of the stria terminalis. Although several reports suggested that NPW is implicated in the regulation of energy homeostasis and nociception, the precise physiological role of NPW has remained unclear. In this study, we examined distribution of NPW messenger RNA and NPW immunoreactivity in the adult rat brain. NPW-immunoreactive (ir) cells were detected in the ventral tegmental area, periaqueductal gray, and Edinger-Westphal nucleus. NPW-ir fibers were observed in several brain regions, including the lateral septum, bed nucleus of the stria. terminalis, dorsomedial and posterior hypothalamus, central amygdaloid nucleus, CA1 field of hippocampus, interpeduncular nucleus, inferior colliculus, lateral parabrachial nucleus, facial nucleus, and hypoglossal nucleus. NPW-ir fibers were most abundantly observed in the central amygdaloid nucleus and the bed nucleus of the stria. terminalis, which are regions implicated in fear and anxiety. These results suggest that NPW might be involved in the regulation of stress and emotive responses, especially in fear and anxiety-related physiological and behavioral functions. (c) 2006 Elsevier B.V. All rights reserved.