Neonatal administration of prolactin antiserum alters the developmental pattern of T- and B-lymphocytes in the thymus and spleen of BALB/c female mice.
Neonatal administration of prolactin antiserum alters the developmental pattern of T- and B-lymphocytes in the thymus and spleen of BALB/c female mice.
复制标题
新生儿给予催乳素抗血清会改变 BALB/c 雌性小鼠胸腺和脾脏中 T 淋巴细胞和 B 淋巴细胞的发育模式。
DOI：
10.1073/pnas.85.19.7404
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发表时间：
1988
影响因子：
11.1
通讯作者：
Bern,HA
中科院分区：
文献类型：
--
作者：
Russell,DH;Mills,KT;Talamantes,FJ;Bern,HA
我们评估了新生儿给予小鼠催乳素 (PRL) 抗血清对雌性 BALB/c 小鼠胸腺和脾脏中 T 淋巴细胞和 B 淋巴细胞发育表达的影响。新生雌性小鼠在第 1、2、3 天皮下注射 50 微升 PRL 抗血清或正常兔血清。在新生第 5 天，PRL 抗血清治疗组胸腺和脾脏中 Thy-1.2 和 L3T4 阳性细胞数量显着增加（P 小于 0.05）。在接受 PRL 抗血清的小鼠和注射溴隐亭（一种抑制垂体前叶释放 PRL 的多巴胺激动剂）的小鼠中，第 8 天和第 14 天，胸腺中 Thy-1.2 和 L3T4 阳性细胞的增加也可检测到。在新生儿第 21、28 和 32 天，胸腺中 Thy-1.2、Ly-2（以前称为 Lyt-2）或 L3T4 抗原阳性细胞的百分比没有显着差异。然而，溴隐亭治疗组和 PRL 抗血清治疗组（第 14 天除外）的 Thy-1.2 和 L3T4 阳性脾细胞百分比在所有监测时间均显着增加。此外，PRL 抗血清治疗组中 IgG 阳性的脾细胞百分比在第 8-28 天显着增加，但在新生儿第 32 天没有显着增加。在已知含有 PRL 受体的组织中，新生儿给予PRL抗血清或溴隐亭会导致脾脏和肝脏湿重显着改变，而对胸腺、心脏和肾脏没有显着影响。垂体植入还导致伴刀豆球蛋白 A 和脂多糖刺激的胸苷掺入 45 日龄雌性小鼠制备的小鼠脾淋巴细胞中的量显着增加。这些数据将 PRL 作为成人淋巴细胞功能的免疫调节剂的作用扩展到在小鼠胸腺和脾脏中 T 淋巴细胞群和 B 淋巴细胞群的发育表达中的作用。
We have evaluated the effect of neonatal administration of mouse prolactin (PRL) antiserum on the developmental expression of T- and B-lymphocytes in the thymus and spleen of female BALB/c mice. Newborn female mice were injected subcutaneously with a 50-microliters aliquot of PRL antiserum or normal rabbit serum on days 1, 2, and 3. On neonatal day 5, the PRL antiserum-treated group had a significantly (P less than 0.05) increased population of cells in the thymus and the spleen that were positive for Thy-1.2 and for L3T4. Increases in Thy-1.2- and L3T4-positive cells in the thymus were detectable also on days 8 and 14 in mice that received the PRL antiserum and in mice injected with bromocriptine, a dopamine agonist that inhibits PRL release from the anterior pituitary. On neonatal days 21, 28, and 32, there were no significant differences in the percentage of cells positive for Thy-1.2, Ly-2 (formerly Lyt-2), or L3T4 antigens in the thymus. However, there were significant increases in the percentage of Thy-1.2- and L3T4-positive spleen cells in the bromocriptine-treated group at all times monitored and in the PRL antiserum-treated group except on day 14. In addition, the percentage of splenocytes that were positive for IgG was significantly increased in the PRL antiserum-treatment group on days 8-28, although not on neonatal day 32. Of tissues known to contain PRL receptors, neonatal administration of PRL antiserum or bromocriptine resulted in a significant alteration in the wet weight of spleen and liver, with no significant effect in thymus, heart, and kidney. Pituitary implants also resulted in a significant increase in both concanavalin A- and lipopolysaccharide-stimulated thymidine incorporation into murine splenic lymphocytes prepared from 45-day-old female mice. These data extend the role of PRL as an immunomodulator of adult lymphocyte function to a role in the developmental expression of T- and B-lymphocyte populations in the thymus and spleen of mice.