Pericentromere tension is self-regulated by spindle structure in metaphase.
Pericentromere tension is self-regulated by spindle structure in metaphase.
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DOI：
10.1083/jcb.201312024
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发表时间：
2014-05-12
期刊：
影响因子：
--
通讯作者：
Gardner MK
中科院分区：
文献类型：
--
作者：
Chacón JM;Mukherjee S;Schuster BM;Clarke DJ;Gardner MK
酵母中的着丝粒周围张力是相当大的，并且通过纺锤体结构的调整由中期有丝分裂纺锤体进行严格的自我调节。在细胞分裂过程中，有丝分裂纺锤体由细胞构建，并在最终分离成子细胞之前对齐和拉伸复制的姐妹染色体。中期期间近着丝粒染色质的伸展被认为会产生一种基于张力的信号，促进染色体的正确分离。然而，目前尚不清楚是否有丝分裂纺锤体积极保持一个设定点张力大小，正确连接的姐妹染色体，以促进强大的机械化学检查点信号。通过成像和跟踪的热运动的pericentromeric荧光标记在酿酒酵母，我们测量pericentromere刚度，然后使用刚度测量，以定量评估张力产生的中期在野生型细胞和突变体染色体结构破坏的pericentromere拉伸。我们发现，在酵母中的pericentromere张力是大量的（4-6 pN），并严格自我调节的有丝分裂纺锤体：通过调整纺锤体结构，细胞保持野生型张力的幅度，即使当pericentromere刚度被破坏。
Pericentromere tension in yeast is substantial and is tightly self-regulated by the metaphase mitotic spindle through adjustments in spindle structure. During cell division, a mitotic spindle is built by the cell and acts to align and stretch duplicated sister chromosomes before their ultimate segregation into daughter cells. Stretching of the pericentromeric chromatin during metaphase is thought to generate a tension-based signal that promotes proper chromosome segregation. However, it is not known whether the mitotic spindle actively maintains a set point tension magnitude for properly attached sister chromosomes to facilitate robust mechanochemical checkpoint signaling. By imaging and tracking the thermal movements of pericentromeric fluorescent markers in Saccharomyces cerevisiae, we measured pericentromere stiffness and then used the stiffness measurements to quantitatively evaluate the tension generated by pericentromere stretch during metaphase in wild-type cells and in mutants with disrupted chromosome structure. We found that pericentromere tension in yeast is substantial (4–6 pN) and is tightly self-regulated by the mitotic spindle: through adjustments in spindle structure, the cell maintains wild-type tension magnitudes even when pericentromere stiffness is disrupted.
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影响因子：
2.8
作者：
Chacon, Jeremy M.;Gardner, Melissa K.
通讯作者：
Gardner, Melissa K.
影响因子：
21.3
作者：
Bakhoum, Samuel F.;Thompson, Sarah L.;Manning, Amity L.;Compton, Duane A.
通讯作者：
Compton, Duane A.
影响因子：
21.3
作者：
Gupta, Mohan L., Jr.;Carvalho, Pedro;Pellman, David
通讯作者：
Pellman, David
DOI：
10.1083/jcb.200910085
发表时间：
2010-03-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kawamura R;Pope LH;Christensen MO;Sun M;Terekhova K;Boege F;Mielke C;Andersen AH;Marko JF
通讯作者：
Marko JF
影响因子：
3.3
作者：
Gardner, MK;Pearson, CG;Odde, DJ
通讯作者：
Odde, DJ