The expression of β-Defensin-2, IL-22, IL-22R1 and IL-10R2 in rat model of Klebsiella pneumonia and their correlation with histological grades
The expression of β-Defensin-2, IL-22, IL-22R1 and IL-10R2 in rat model of Klebsiella pneumonia and their correlation with histological grades
复制标题
β-防御素-2、IL-22、IL-22R1和IL-10R2在肺炎克雷伯菌肺炎大鼠模型中的表达及其与组织学分级的关系
DOI：
10.1080/01902148.2020.1725690
复制
发表时间：
2020-03-13
影响因子：
1.7
通讯作者：
Li, Nie
中科院分区：
文献类型：
--
作者：
Fan, Jianyong;Luo, Yuan;Li, Nie
背景和目标：肺炎克雷伯氏菌是导致医院获得性肺炎克雷伯氏菌感染的最常见的机会致病菌。克雷伯菌肺炎具有快速进展的临床过程和多药耐药（MDR）。寻找有效的生化标志物对提高克雷伯菌肺炎的早期诊断和治疗至关重要。本研究的目的是：1）研究β-防御素-2（r β D2）、IL-22、IL-22 R1和IL-10 R2在肺炎克雷伯菌感染大鼠中的表达及其与肺炎克雷伯菌组织学分级的关系。方法和材料：将50只SPF级雄性SD大鼠随机分为两组：对照组（生理盐水）和肺炎组（肺炎克雷伯菌）。肺炎）。分别于感染后1h、12 h、1d、3d、5d处死动物。通过组织病理学观察和病原体鉴定，评估肺炎的严重程度和性质。RT-qPCR法检测r β D2、IL-22、IL-22 R1和IL-10 R2的mRNA表达。Western blot法检测大鼠肺组织中r β D2的表达，ELISA法检测大鼠血清中IL-22的水平。结果：肺组织病理学检查及细菌计数证实大鼠肺炎模型建立成功。肺炎组小鼠r β D2、IL-22、IL-22 R1和IL-10 R2基因表达均显著高于健康对照组（P < 0.05）。r β D2的表达与肺炎克雷伯菌的组织学分级和IL-22水平相关。RT-qPCR结果显示，在大鼠肺炎模型中，IL-22 R1的表达峰值出现早于IL-10 R2。结论：在大鼠肺炎克雷伯菌模型中，r β D2和IL-22的表达在早期即显著升高，提示IL-22和r β D2可能作为早期诊断肺炎克雷伯菌的潜在生物标志物。
Backgrounds and Aims: Klebsiella pneumoniae represents the most common opportunistic pathogen contributing to Klebsiella pneumonia in hospital-acquired infections. Klebsiella pneumonia has a rapidly progressive clinical course and multi-drug resistant (MDR). Identification of the effective biochemical markers is crucial for improving early diagnosis and treatment of Klebsiella pneumonia. The aims of our study are to 1) investigate the expression of beta-Defensin-2(r beta D2), IL-22, IL-22R1 and IL-10R2 in Klebsiella pneumonia-infected rats and 2) their association with the histological grades of Klebsiella pneumonia. Methods and Materials: Fifty specific pathogen free (SPF) male SD rats were randomly divided into two groups: control group (treated with normal saline) and pneumonia group (treated with K. pneumoniae). All animals were sacrificed 1 h, 12 h, 1 d, 3 d, 5 d post infection. The severity and property of pneumonia was evaluated by histopathologic observation and pathogen identification. The mRNA expression of r beta D2, IL-22, IL-22R1 and IL-10R2 was measured by RT-qPCR assay. The expression of r beta D2 in rat lung tissue was determined by Western blot analysis, and the level of IL-22 in rat serum was determined by ELISA. Results: Histopathologic examination and bacterial counting of lung tissues confirmed the successful establishment of rat pneumonia model. The gene expression of r beta D2, IL-22, IL-22R1 and IL-10R2 in pneumonia rats were significantly higher than those in healthy control mice (P < 0.05). The expression of r beta D2 was correlated with histological grades of Klebsiella pneumonia and the level of IL-22. RT-qPCR results showed that the peak expression of IL-22R1 appeared earlier than IL-10R2 in rat pneumonia model. Conclusions: The expression of r beta D2 and IL-22 was increased significantly at early stage in rat Klebsiella pneumonia model, suggesting that IL-22 and r beta D2 might serve as potential biomarkers for the early diagnosis of Klebsiella pneumonia.