Karrikins-induced accumulation of Salvianolic acid B is mediated by calcium–calmodulin, brassinolide and jasmonic acid in Salvia miltiorrhiza
Karrikins-induced accumulation of Salvianolic acid B is mediated by calcium–calmodulin, brassinolide and jasmonic acid in Salvia miltiorrhiza
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DOI：
10.1016/j.sajb.2020.03.033
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发表时间：
2020-08
影响因子：
3.1
通讯作者：
Hui Sun;Xu Zixin-;Jie Zhou;Lanping Guo
中科院分区：
文献类型：
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作者：
Hui Sun;Xu Zixin-;Jie Zhou;Lanping Guo
研究了烟源karrikins（KAR 1）对丹参毛状根内源信号分子Ca 2 +-钙调素（CaM）、油菜素（BL）、茉莉酸（JA）及次生代谢产物丹参酸B（SA B）含量的影响。结果表明，Ca ~（2+）-CaM、BL和JA的爆发是S.丹参对KAR 1有反应。外源CaCl 2和BL处理显著提高了丹参毛状根中JA的含量，而JA抑制剂水杨基异羟肟酸/没食子酸正丙酯（SHAM/PrGall）对Ca 2+和BL的含量没有影响。此外，Ca 2+通道阻断剂LaCl 3（La 3+）和维拉帕米（VERA），CaM拮抗剂[N-（6-aminohexyl）-5-chloro-1-naphthalensulfonamide hydrochloride]（W7）和三氟拉嗪（TFP）预处理显著减少KAR 1诱导的BL积累，而BL抑制剂阿曲立唑（BRz）则减少KAR 1诱导的Ca 2+产生。这表明Ca ~（2+）和BL之间存在着平行关系，是抑制JA合成的上游信号分子。外源茉莉酸甲酯（jasmonate methyl，JAMe）可逆转VERA、W7、BRz和SHAM对KAR 1诱导的SAB产生的抑制作用，而外源CaCl 2和BL则无此作用。这表明Ca ~（2+）-CaM，BL介导了KAR_1诱导的丹参毛状根SAB的增加，并依赖于JA的产生。本研究有助于我们了解Ca ~（2+）-CaM、BL、JA对KAR 1诱导丹参毛状根SAB积累的调控机制。
The effects of smoke-derived karrikins (KAR1) on the contents of endogenous signal molecules including (Ca2+)–calmodulin (CaM), brassinolide (BL) and Jasmonic acid (JA) and secondary metabolite Salvianolic acid B (SAB) inSalvia miltiorrhizahairy roots have been investigated. The results showed that the burst of Ca2+–CaM, BL and JA were early responses ofS. miltiorrhizaresponding to KAR1. Treatments with exogenous CaCl2and BL dramatically improved the level of JA, while JA inhibitor salicylhydroxamic acid/n-propylgallate (SHAM/PrGall) did not influence levels of Ca2+and BL inS. miltiorrhizahairy roots. Furthermore, pretreatments with the Ca2+channel blockers LaCl3(La3+) and Verapamil (VERA), and CaM antagonists [N-(6-amin-ohexyl)-5-chloro-1-naphthalene sulfonamide hydrochloride] (W7) and trifluoperazine (TFP) substantially reduced KAR1-induced BL accumulation, while the Ca2+production induced by KAR1was reduced by BL inhibitor Brassinazole (BRz). It suggested that Ca2+and BL seem to have a parallel relationship and act as upstream signaling molecules to medicate the production of JA. The inhibitory effects of VERA, W7, BRz and SHAM toward SAB production induced by the KAR1could be reversed by exogenous jasmonate methyl (JAMe), while the exogenous CaCl2and BL had no similar effects. It proved that Ca2+–CaM, BL mediated the KAR1-induced increase of SAB dependent on the generation of JA inS. miltiorrhizahairy roots. This study help us understand the regulatory mechanism of Ca2+–CaM, BL, JA on KAR1-induced accumulation of SAB inS. miltiorrhizahairy roots.