Low-dose radiation does not induce proliferation in tumor cells in vitro and in vivo
Low-dose radiation does not induce proliferation in tumor cells in vitro and in vivo
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DOI：
10.1667/rr1132.1
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发表时间：
2008-10-01
影响因子：
3.4
通讯作者：
Wang, Guanjun
中科院分区：
文献类型：
--
作者：
Jiang, Hongyu;Xu, Ying;Wang, Guanjun
我们已经证明，小鼠暴露于低剂量辐射（75 mGy）刺激骨髓细胞增殖和外周动员（Li等人，Exp.血液32，1088-1096，2004）。目前还不清楚低剂量辐射诱导的这种刺激效应是否也会发生在肿瘤细胞中。在本研究中，两个白血病细胞系和五个实体瘤细胞系的细胞与四个正常的人类细胞系一起被用来确定是否暴露于低剂量辐射（25至200 mGy的X射线）可以引起刺激效应油细胞增殖。在正常细胞系中发现了刺激效应，但在两个白血病细胞系和五个实体瘤细胞系中没有发现对低剂量辐射的反应。在低剂量近距离照射后不同时间，通过流式细胞术检查这些细胞的细胞周期变化和细胞死亡，未发现任何可归因于低剂量辐射对肿瘤细胞和正常细胞之间的细胞增殖的不同影响的变化。进一步证明低剂量辐射对体内肿瘤细胞无刺激作用。将两种实体瘤细胞系的细胞植入裸鼠中。与没有低剂量辐射暴露的荷瘤小鼠相比，在裸鼠或荷瘤小鼠中植入肿瘤细胞之前在体外暴露于低剂量辐射（75 mGy X射线）不会刺激肿瘤生长。这些结果表明，低剂量辐射刺激正常细胞的生长，但不是白血病和实体瘤细胞在体外，也不刺激实体瘤细胞在体内的生长。(C)2008年，辐射研究协会。
We have demonstrated that exposure of mice to low-dose radiation (75 mGy) stimulated bone marrow cell proliferation and peripheral mobilization (Li et al, Exp. Hematol. 32, 1088-1096, 2004). It is unclear whether such stimulating effects induced by low-dose radiation can also occur in tumor cells. In the present study, cells of two leukemia cell lines and five solid tumor cell lines together with four normal human cell lines were used to determine whether exposure to low-dose radiation (25 to 200 mGy X rays) can cause a stimulating effect oil cell proliferation. A stimulating effect was found in the normal cell lines but not in the two leukemia and five solid tumor cell lines in response to low-dose radiation exposure in vitro. Examination of cell cycling changes and cell death for these cells by flow cytometry at different times after low-close irradiation did not identify any changes attributable to the distinct effects of low-dose radiation on cell proliferation between tumor and normal cells. To provide further evidence for the absence of low-dose radiation-induced stimulating effects in tumor cells in vivo. cells of two solid tumor cell lines were implanted in nude mice. Exposure of tumor cells in vitro before implantation in nude mice or of tumor-bearing mice to low-dose radiation (75 mGy X rays) did not stimulate tumor growth compared to the tumor-bearing mice without low-dose radiation exposure. These results suggest that low-dose radiation stimulates growth of normal cells but not of leukemia and solid tumor cells in vitro and also does not stimulate growth of solid tumor cells in vivo. (C) 2008 by Radiation Research Society.