Determination of alternariol and alternariol methyl ether in apple juice using solid-phase extraction and high-performance liquid chromatography
Determination of alternariol and alternariol methyl ether in apple juice using solid-phase extraction and high-performance liquid chromatography
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DOI：
10.1016/0021-9673(95)01200-1
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发表时间：
1996-04-19
影响因子：
4.1
通讯作者：
Scott, PM
中科院分区：
文献类型：
--
作者：
Delgado, T;GomezCordoves, C;Scott, PM
目前的工作描述了一种测定苹果汁中交链孢醇 (AGH) 和交链孢醇甲醚 (AME) 的新方法，使用固相萃取 (SPE) 柱提取和净化样品以进行高效液相色谱 (HPLC)。加标样品的色谱图表明，两种毒素都可以轻松检测而不会受到干扰，并且 AOH (82.8 +/- 7.4%) 和 AME (91.9 +/- 6.1%) 具有良好的回收率，检测限分别低至 1.6 和 0.7 μg/l。
The present work describes a new method for determination of alternariol (AGH) and alternariol methyl ether (AME) in apple juice using solid-phase extraction (SPE) columns for extraction and cleanup of samples for high-performance liquid chromatography (HPLC). Chromatograms of spiked samples show that both toxins can be easily detected without interferences, and good recoveries for AOH (82.8 +/- 7.4%) and AME (91.9 +/- 6.1%) with detection limits as low as 1.6 and 0.7 mu g/l, respectively, were obtained.