Chromosome aberrations induced in human lymphocytes by neutron irradiation.
Chromosome aberrations induced in human lymphocytes by neutron irradiation.
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中子辐照在人淋巴细胞中诱导染色体畸变。
DOI：
10.1080/09553007614550181
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
A. Edwards
中科院分区：
文献类型：
--
作者：
D. Lloyd;R. J. Purrott;G. Dolphin;A. Edwards
对平均能量为0-7、0-9、7-6和14-7 MeV的中子谱，给出了人淋巴细胞不稳定染色体畸变率的体外剂量-反应曲线。像差产额符合二次函数Y=αD+βD 2，符合像差形成的单道和双道模型。然而，对于高LET辐射，对应于单道损伤的产额的线性分量占主导地位，并且随着LET的增加，这一项变得更加主导，因此对于裂变谱中子，这种关系是线性的，Y=αD。在低剂量时，例如辐射工作人员所接受的剂量，限制了r.b.e。相对于~(60)Co伽马辐射，得到的值在13到47之间。在放射治疗中使用的高剂量时，数值要低得多；在200拉德的当量伽马辐射下，数值在2-7到8之间。两套r.b.e。数值与径迹平均LET有很好的相关性，但与剂量平均LET不相关。当没有像差的细胞数量与辐射剂量作图时，得到的曲线形状类似于具有类似中子谱的常规细胞存活实验的曲线。本研究得到的DO值与其他细胞系统的DO值接近。
In vitro dose--response curves of unstable chromosome aberrations in human lymphocytes have been obtained for neutron spectra of mean energies 0-7, 0-9, 7-6 and 14-7 MeV. The aberration yields have been fitted to the quadratic function Y = alphaD + betaD2, which is consistent with the single-track and two-track model of aberration formation. However with high-LET radiation, the linear component of yield, corresponding to damage caused by single tracks, predominants, and this term becomes more dominant with increasing LET, so that for fission spectrum neutrons the relationship is linear, Y = alphaD. At low doses, such as those recieved by radiation workers, limiting r.b.e. values between 13 and 47 are obtained relative to 60Co gamma-radiation. At higher doses, as used in radiotherapy, the values are much lower; ranging from 2-7 to 8 at 200 rad of equivalent gamma-radiation. Both sets of r.b.e. values correlate well with track-averaged LET but not with dose-averaged LET. When the numbers of cells without aberrations are plotted against radiation dose, curves are obtained which are similar in shape to those for conventional cell-survival experiments with comparable neutron spectra. The Do values obtained in the present study are close to those from other cell system.