Irradiation induces increased production of haemopoietic and proinflammatory cytokines in the mouse lung
Irradiation induces increased production of haemopoietic and proinflammatory cytokines in the mouse lung
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DOI：
10.1080/09553000110104614
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发表时间：
2002-04-01
影响因子：
2.6
通讯作者：
Vacek, A
中科院分区：
文献类型：
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作者：
Fedorocko, P;Egyed, A;Vacek, A
目的：研究小鼠辐照后细胞因子的表达，主要是在肺组织中，但也在其他组织中。材料与方法：将ICR品系小鼠全身（单侧）暴露于320gy的Co-60 γ射线下。采用GM-CFC生物测定法测定小鼠肺条件介质（LCM）、其他非造血和造血器官以及血清的集落刺激活性（CSA）。ELISA法检测小鼠血清中GM-CSF、IL-6和tnf - α蛋白的表达。结果：在肺的条件培养基中检测到最大的CSA，并以剂量和时间依赖性的方式诱导，在致死剂量为9 Gy的照射后39天达到峰值。体外辐照剂量为9gy的肺制备的条件培养基没有显示出CSA的增加。然而，来自受辐射小鼠的肺条件培养基被发现产生CSA，而来自正常小鼠或受辐射小鼠的血清则不会产生这种效果。在IL-3浓度不理想的情况下，血清中CSA显著增加，这意味着它们包含了共刺激活性（CoSA）。结果表明，辐射暴露增加了LCM中GM-CSF和tnf - α蛋白水平，但不影响IL-6的产生。相比之下，辐照后血清IL-6和tnf - α水平升高，但未检测到GM-CSF的产生。结论：全身照射可增强肺及其他造血和非造血器官的CSA。CSA的升高与肺造血细胞因子和促炎细胞因子的升高有关。
Purpose: To investigate cytokine expression following irradiation of mice, predominantly in lung tissue but also in selected other tissues.Materials and methods: Mice of strain ICR were whole-body (unilaterally) exposed to 3 20 Gy of Co-60 gamma-rays. Colony-stimulating activity (CSA) of lung-conditioned media (LCM), and also other non-haemopoietic and haemopoietic organs, and blood serum of mice was assayed using a GM-CFC bioassay. The production of GM-CSF, IL-6 and TNF-alpha protein in LCM and sera was determined by an ELISA method.Results: Greatest CSA was detected in conditioned media from the lungs and was induced in a dose- and time-dependent fashion, peaking at 3 9 days after irradiation with a lethal dose of 9 Gy. Conditioned medium prepared from lungs that had been irradiated with a dose of 9 Gy in vitro did not exhibit an increase in CSA. However, whereas the lung-conditioned medium from irradiated mice was found to produce CSA, sera from normal or irradiated mice did not lead to this effect. A significant increase in CSA in sera was observed in the presence of a suboptimal concentration of IL-3, implying that they comprise the co-stimulatory activity (CoSA). The results showed that radiation exposure increased GM-CSF and TNF-alpha protein levels but did not affect IL-6 production in LCM. In contrast, IL-6 and TNF-alpha protein levels in serum were increased after irradiation but no GM-CSF production could be detected.Conclusion: Whole-body irradiation enhances CSA in lungs as well as in other haemopoietic and non-haemopoietic organs. The increase of CSA correlates with increased levels of haemopoietic and proinflammatory cytokines in lung.