LOCOMOTION-INDUCING SITES IN THE VICINITY OF THE PEDUNCULOPONTINE NUCLEUS
LOCOMOTION-INDUCING SITES IN THE VICINITY OF THE PEDUNCULOPONTINE NUCLEUS
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DOI：
10.1016/0361-9230(87)90208-5
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发表时间：
1987-06-01
影响因子：
3.8
通讯作者：
WOODWARD, DJ
中科院分区：
文献类型：
--
作者：
GARCIARILL, E;HOUSER, CR;WOODWARD, DJ
通过对脑桥中脑被盖区域施加低幅度电流脉冲，在丘前大鼠的跑步机上诱导受控运动，从而在生理学上识别中脑运动区（MLR）。使用两种已知标记桥脚核（PPN）神经元的技术-胆碱乙酰转移酶（ChAT）免疫细胞化学或烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-心肌黄酶组织化学对相同的大脑进行处理。运动诱导位点的组织学重建位于 ChAT 或 NADPH 心肌黄酶标记的细胞组内或附近。 PPN 的三维重建用于可视化 PPN 神经元聚集体内低阈值运动诱导刺激位点的共定位。这些发现进一步支持了 PPN 是国土资源部一部分的观点。提出了一个理论框架来解释该领域各个研究方向的结果。
The mesencephalic locomotor region (MLR) was identified physiologically by inducing controlled locomotion on a treadmill in the precollicular rat following application of low amplitude current pulses to areas of the pontomesencephalic tegmentum. The same brains were processed using either of two techniques known to label neurons of the pedunculopontine nucleus (PPN)-choline acetyltransferase (ChAT) immunocytochemistry or nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry. Histological reconstruction of locomotion-inducing sites were localized within or adjacent to ChAT or NADPH-diaphorase labeled cell groups. Three dimensional reconstructions of the PPN were used to visualize the colocalization of low threshold locomotion-inducing stimulation sites within PPN neuronal aggregates. These findings lend further support to the suggestion that the PPN is part of the MLR. A theoretical framework is proposed to account for results derived from various lines of research on this area.