Peripheral somatosensory fMRI in mouse at 11.7 T
Peripheral somatosensory fMRI in mouse at 11.7 T
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DOI：
10.1002/nbm.709
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发表时间：
2001-08-01
影响因子：
2.9
通讯作者：
Dubowitz, DJ
中科院分区：
文献类型：
--
作者：
Ahrens, ET;Dubowitz, DJ
研究了在11.7T下使用BOLD对比剂对麻醉小鼠进行极高分辨率体感功能磁共振成像的可行性。将躯体感觉刺激施加到α-氯蔗糖麻醉的小鼠的后肢，导致躯体感觉皮层和大静脉的稳健（p < 4 × 10（-3））BOLD变化。皮层MR信号的调制百分比超过7%。还进行了人工调节吸入氧张力的实验;结果显示小鼠大脑中存在大的、不均匀的BOLD对比度变化。此外，评价了11.7 T时灰质中的T-1、T-2和T-2* 值。讨论了BOLD功能磁共振成像在小鼠中枢神经系统的灵敏度限制。这些方法显示出在CNS损伤和疾病的小鼠模型中评估神经功能的希望。版权所有（C）2001约翰威利父子有限公司
The feasibility of performing extremely-high resolution somatosensory fMRI in anesthetized mice using BOLD contrast at 11.7 T was investigated. A somatosensory stimulus was applied to the hindlimb of an alpha -chlorolose anesthetized mouse resulting in robust (p < 4 x 10(-3)) BOLD changes in somatosensory cortex and large veins. Percentage modulation of the MR signal in cortex exceeded 7%. Experiments that artificially modulated the inspired oxygen tension were also conducted; the results revealed large, heterogeneous, BOLD contrast changes in the mouse brain. In addition, T-1, T-2, and T-2* values in gray matter at 11.7 T were evaluated. Discussion of the sensitivity limitations of BOLD fMRI in the tiny mouse central nervous system is presented. These methods show promise for the assessment of neurological function in mouse models of CNS injury and disease. Copyright (C) 2001 John Wiley & Sons, Ltd.