Mountain grown ginseng induces apoptosis in HL-60 cells and its mechanism have little relation with TNF-α production
Mountain grown ginseng induces apoptosis in HL-60 cells and its mechanism have little relation with TNF-α production
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DOI：
10.1142/s0192415x07004710
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发表时间：
2007-01-01
影响因子：
5.7
通讯作者：
Kim, Hyung-Min
中科院分区：
文献类型：
--
作者：
Koo, Hyun-Na;Jeong, Hyun-Ja;Kim, Hyung-Min
人参根是东方国家最受欢迎的天然滋补品之一。人参生长在野外，在深山中，被称为Sansam（山地人参，MGG）。MGG为五加科人参属植物。在这项研究中，我们研究了MGG对人早幼粒白血病细胞HL-60的细胞毒性，诱导凋亡及其作用的可能途径。通过细胞凋亡分析，我们发现MGG是一种强有力的凋亡诱导剂，但它对人外周血单个核细胞的影响较小。半胱天冬酶-3抑制剂Z-DEVD-FMK可部分阻断MGG处理的细胞中的半胱天冬酶-3激活和随后的凋亡性细胞死亡。MGG还抑制caspase-8活性。为了确定MGG诱导的细胞凋亡是否参与肿瘤坏死因子-α（TNF-α）分泌，通过酶联免疫吸附测定（ELISA）方法定量TNF-α分泌。出乎意料的是，与对照组相比，MGG显著降低了TNF-α的分泌。这些结果表明MGG诱导的HL-60细胞毒性与TNF-α的分泌关系不大。此外，MGG与rIFN-γ协同增加小鼠腹腔巨噬细胞中一氧化氮（NO）的产生。总之，我们的数据表明，MGG是一个强大的诱导HL-60细胞凋亡，这些能力可以用于临床治疗癌症。
The root of ginseng is one of the most popular natural tonics in Oriental countries. Ginseng grown in the wild, deep in the mountains, is known as Sansam (mountain grown ginseng, MGG). MGG belongs to Araliaceae and Panax. In this study, we investigated the effects of MGG on the cytotoxicity, induction of apoptosis and the putative pathways of its actions in human promyelocytic leukemia cells, HL-60. Using apoptosis analysis, we found that MGG is a potent inducer of apoptosis, but it has less effect on human peripheral blood mononuclear cells. Caspase-3 activation and subsequent apoptotic cell death in MGG-treated cells were partially blocked by the caspase-3 inhibitor, Z-DEVD-FMK. MGG also inhibited the caspase-8 activity. To determine whether MGG-induced apoptosis is involved in tumor necrosis factor-alpha (TNF-alpha) secretion, TNF-alpha secretion was quantified by enzyme-linked immunosorbent assay (ELISA) method. Unexpectedly, MGG significantly decreased the TNF-alpha secretion compared to the control. These results suggest that MGG-induced cytotoxicity have little relation with the secretion of TNF-alpha in HL-60 cells. Furthermore, MGG with rIFN-gamma synergistically increased nitric oxide (NO) production in mouse peritoneal macrophages. Taken together, our data indicate that MGG is a potent inducer of apoptosis on HL-60 cells and these abilities could be used clinically for the treatment of cancer.