Phospholipase C beta 4 is involved in modulating the visual response in mice
Phospholipase C beta 4 is involved in modulating the visual response in mice
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DOI：
10.1073/pnas.93.25.14598
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发表时间：
1996-12-10
影响因子：
11.1
通讯作者：
Wu, DQ
中科院分区：
文献类型：
--
作者：
Jiang, HP;Lyubarsky, A;Wu, DQ
G蛋白调节的磷脂酶C（PLC）β 4在视网膜、外侧膝状体核和上级丘中的表达暗示PLC β 4可能在哺乳动物视觉过程中起作用。产生缺乏PLC β 4的小鼠品系并研究PLC β 4在小鼠视觉功能中的生理学意义，使用穿梭机进行的行为测试表明，缺乏PLC β 4的小鼠的视觉处理能力受损，而它们的听觉能力没有缺陷。此外，PLC β 4缺失小鼠显示其视网膜电图的视杆细胞a-和b-波分量的最大振幅相对于其同窝对照降低4倍，然而，来自单视杆光感受器的记录没有揭示PLC β 4-无效和野生型同窝出生仔之间的显著差异，用光学显微镜检查的视网膜也没有任何明显的差异。虽然行为和视网膜电图结果表明PLC β 4在哺乳动物视觉信号处理中起重要作用，但分离的视杆记录显示很少或没有明显的缺陷，这表明PLC β 4缺陷对视杆信号通路的影响发生在初始光转导级联后的某个阶段，并且可能需要视杆和其他视网膜细胞之间的细胞-细胞相互作用。
Expression of G protein-regulated phospholipase C (PLC) beta 4 in the retina, lateral geniculate nucleus, and superior colliculus implies that PLC beta 4 may play a role in the mammalian visual process, A mouse line that lacks PLC beta 4 was generated and the physiological significance of PLC beta 4 in murine visual function was investigated, Behavioral tests using a shuttle bos demonstrated that the mice lacking PLC beta 4 were impaired in their visual processing abilities, whereas they showed no deficit in their auditory abilities, In addition, the PLC beta 4-null mice showed 4-fold reduction in the maximal amplitude of the rod a- and b-wave components of their electroretinograms relative to their littermate controls, However, recording from single rod photoreceptors did not reveal an significant differences between the PLC beta 4-null and wild-type littermates, nor were there any apparent differences in retinas examined with light microscopy. While the behavioral and electroretinographic results indicate that PLC beta 4 plays a significant role in mammalian visual signal processing, isolated rod recording shows little or no apparent deficit, suggesting that the effect of PLC beta 4 deficiency an the rod signaling pathway occurs at some stage after the initial phototransduction cascade and may require cell-cell interactions between rods and other retinal cells.