Enhanced fermentative hydrogen production from industrial wastewater using mixed culture bacteria incorporated with iron, nickel, and zinc-based nanoparticles
Enhanced fermentative hydrogen production from industrial wastewater using mixed culture bacteria incorporated with iron, nickel, and zinc-based nanoparticles
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DOI：
10.1016/j.watres.2018.12.043
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发表时间：
2019-03-15
期刊：
影响因子：
12.8
通讯作者：
Tawfik, Ahmed
中科院分区：
文献类型：
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作者：
Elreedy, Ahmed;Fujii, Manabu;Tawfik, Ahmed
本研究评估了利用混合培养细菌（MCB）与单个纳米粒子（NP）（即赤铁矿（α-Fe2O3）、氧化镍（NiO）和氧化锌（ZnO）、双纳米粒子（α-Fe2O3 + NiO、α-Fe2O3 + ZnO和NiO + ZnO）以及多纳米粒子（α-Fe2O3 + NiO + ZnO）结合的制氢效率(HP) 来自含有单乙二醇 (MEG) 的工业废水。当MCB单独添加α-Fe2O3（200 mg/L）、NiO（20 mg/L）和ZnO NPs（10 mg/L）时，HP分别显着提高41%、30%和29%。此外，与 MEG 代谢相关的关键酶，如乙醇脱氢酶 (ADH)、乙醛脱氢酶 (ALDH) 和氢化酶 (hyd)，在培养基中迅速且显着增强。 NiO 和 ZnO NPs 分别显着促进 ADH 和 ALDH 活性，而 α-Fe2O3 对 hyd 活性表现出卓越的影响。最大氢气生产率伴随着较高的乙酸产量和较低的残留乙醛和乙醇。当使用双重和多重纳米粒子时，使用补充有单独的 NiO (20 mg/L) 和 ZnO NPs (10 mg/L) 的 MCB 的 HP 进一步提高了 8.0%-14%；当使用多个 NP 时记录了最高的 HP。此外，纳米粒子的掺入导致梭菌目（属于梭菌科；> 83%）的相对丰度大幅增加。总体而言，这项研究提供了关于纳米粒子对 MEG 污染废水制氢影响的重要见解。 (C) 2018 Elsevier Ltd. 保留所有权利。
The present study assessed the efficiency of utilizing mixed culture bacteria (MCB) incorporated with individual nanoparticles (NPs), i.e., hematite (alpha-Fe2O3), nickel oxide (NiO), and zinc oxide (ZnO), dual NPs (alpha-Fe2O3 + NiO, alpha-Fe2O3 + ZnO, and NiO + ZnO), and multi-NPs (alpha-Fe2O3 + NiO + ZnO) for hydrogen production (HP) from industrial wastewater containing mono-ethylene glycol (MEG). When MCB was individually supplemented with alpha-Fe2O3(200 mg/L), NiO (20 mg/L), and ZnO NPs (10 mg/L), HP improved significantly by 41, 30, and 29%, respectively. Further, key enzymes associated with MEG metabolism, such as alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), and hydrogenase (hyd), were rapidly and substantially enhanced in the medium. NiO and ZnO NPs notably promoted ADH and ALDH activities, respectively, while alpha-Fe2O3 exhibited superior impact on hyd activity. Maximum hydrogen production rate was concomitant with higher acetic acid production and lower residual acetaldehyde and ethanol. HP using MCB supplemented with individual NiO (20 mg/L) and ZnO NPs (10 mg/L) further improved by 8.0%-14% when dual and multi-NPs were used; the highest HP was recorded when multi NPs were used. In addition, NPs incorporation resulted in substantial increase in the relative abundance of Clostridiales (belonging to family Clostridiaceae; > 83%). Overall, this study provides significant insights into the impact of NPs on hydrogen production from MEG-contaminated wastewater. (C) 2018 Elsevier Ltd. All rights reserved.