Genetic Detection of Extended-Spectrum β-Lactamase-Containing Escherichia coli Isolates and Vancomycin-Resistant Enterococci in Fecal Samples of Healthy Children
Genetic Detection of Extended-Spectrum β-Lactamase-Containing Escherichia coli Isolates and Vancomycin-Resistant Enterococci in Fecal Samples of Healthy Children
复制标题
DOI：
10.1089/mdr.2009.0910
复制
发表时间：
2009-09-01
影响因子：
2.6
通讯作者：
Poeta, Patricia
中科院分区：
文献类型：
--
作者：
Guimaraes, Bruno;Barreto, Angela;Poeta, Patricia
2007年10月至2008年2月，在葡萄牙采集了112份健康儿童的粪便样本，并对其进行了超广谱β-内酰胺酶(ESBL)检测，其中包括大肠埃希菌分离株和万古霉素耐药肠球菌(VRE)。112份粪便标本中有3份(2.7%)检出ESBL阳性的大肠埃希菌，并在这些分离株中检测到bla(CTX-M-1)、bla(TEM-52)和bla(SHV-12)基因。含有BLA(TEM52)的分离株表现出对氟喹诺酮类、四环素、甲氧苄氨嘧磺胺甲恶唑和氯霉素的多重耐药表型，在该分离株中除了gyrA(Ser83Leu+Asp87Asn)和ParC蛋白(Ser80Ile)的两个氨基酸变化外，还检测到了sul1、sul3和cmlA基因。ESBL分离株对应于系统群A(1株)、B1(1株)和D(1株)。112份粪便标本中检出13份(11.6%)粪便标本中检出VANA，2份检出VanC-1菌株。在VRE分离物中发现了多样性的抗性基因[tet(M)、tet(L)、erm(B)、aph(3‘)-IIIa、ant(6)-Ia、catA和vat(E)]。这些结果表明，健康儿童的肠道构成了含有ESBL的大肠杆菌和VRE分离株的储存库。
One hundred twelve fecal samples of healthy children were recovered in Portugal during October 2007 and February 2008 and were tested for extended-spectrum beta-lactamases (ESBLs) containing Escherichia coli isolates and vancomycin-resistant enterococci (VRE). Three of the 112 fecal samples (2.7%) harbored ESBL-positive E. coli isolates and the bla(CTX-M-1), bla(TEM-52), and bla(SHV-12) genes were identified in these isolates. The bla(TEM-52)-containing isolate showed a phenotype of multiresistance that included fluoroquinolones, tetracycline, trimethoprim-sulfamethoxazole, and chloramphenicol; sul1, sul3, and cmlA genes were detected in this isolate, in addition to two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC protein (Ser80Ile). The ESBL isolates corresponded to phylogroup A (one isolate), B1 (one isolate), and D (one isolate). vanA-containing Escherichia faecium isolates were detected in 13 of the 112 fecal samples (11.6%), and vanC-1 isolates were found in 2 samples. A diversity of resistance genes [(tet(M), tet(L), erm(B), aph(3 ')-IIIa, ant(6)-Ia, catA, and vat(E)] were found in VRE isolates. These results show that the intestinal tract of healthy children constitutes a reservoir of ESBL-containing E. coli and VRE isolates.