Fluorescence aggregation assay for the protein biomarker mucin 1 using carbon dot-labeled antibodies and aptamers
Fluorescence aggregation assay for the protein biomarker mucin 1 using carbon dot-labeled antibodies and aptamers
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DOI：
10.1007/s00604-014-1386-3
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发表时间：
2015-01-01
影响因子：
5.7
通讯作者：
Yang, Minghui
中科院分区：
文献类型：
--
作者：
Ma, Nan;Jiang, Wentao;Yang, Minghui
我们建立了一种灵敏的蛋白质生物标记物粘蛋白1(MUC1)的荧光分析方法。它是基于官能化碳点(Cd)在MUC1存在下的聚集。尺寸约为2 nm的硫化镉具有较强的蓝绿色本征荧光。抗MUC1抗体和MUC1适配子共价连接到CDs上，CD标记的MUC1抗体和CD标记的适配子与MUC1发生免疫反应，形成夹心结构，伴随着CDs的聚集和荧光的猝灭。荧光的变化与MUC1的浓度在5-100 nm范围内呈线性关系，检测下限为2 nm。该方法用于实际血清样品中MUC1的测定，回收率为94.2%~98.1%。该方法成本低，操作简便。它的范围很广，因为可以想象，它可以扩展到对其他临床感兴趣的蛋白质和生物标记物的分析。
We have developed a sensitive fluorescence assay for the protein biomarker mucin 1 (MUC1). It is based on the aggregation of functionalized carbon dots (CDs) in the presence of MUC1. The CDs with a size of similar to 2 nm display strong blue-green intrinsic fluorescence. Antibodies against MUC1 and the MUC1 aptamer were covalently conjugated to the CDs, and the immunoreaction between CD-labeled antibody to MUC1, and of the CD-labeled aptamer to MUC1 resulted in the formation of a sandwich structure that is accompanied by aggregation of CDs and quenching of fluorescence. The change in fluorescence is linearly related to the MUC1 concentration in the 5 to 100 nM range, with a detection limit at 2 nM. The assay was applied to the determination of MUC1 in (spiked) real serum samples, and good recoveries (ranging from 94.2 to 98.1 %) were obtained. This assay is cost-effective and convenient. It has a wide scope in that it, conceivably, can be extended to assays for other proteins and biomarkers of clinical interest.