The Signaling Peptide EPF2 Controls Asymmetric Cell Divisions during Stomatal Development
The Signaling Peptide EPF2 Controls Asymmetric Cell Divisions during Stomatal Development
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DOI：
10.1016/j.cub.2009.03.069
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发表时间：
2009-05-26
期刊：
影响因子：
9.2
通讯作者：
Gray, Julie E.
中科院分区：
文献类型：
--
作者：
Hunt, Lee;Gray, Julie E.
气孔是植物表皮上控制二氧化碳吸收和水分流失的气孔。它们是全球碳和水循环的主要调节者[1]。几个信号成分调节气孔发育的特点。这些包括推定的分泌肽EPF 1、LRR受体组分TMM和ER以及肽酶SDD 1 [2-4]。我们已经确定了EPF 2，EPF 1相关的肽，表达在气孔谱系的增殖细胞，称为分生组织，并在保卫母细胞，气孔的祖细胞。EPF 2在叶片发育过程中的表达影响成熟叶片上的气孔密度。在没有EPF 2的情况下，过多的细胞进入气孔谱系并产生许多表达气孔谱系报告基因的小表皮细胞，而过表达EPF 2的植物几乎不产生气孔。遗传实验的结果表明，EPF 2调节气孔发育的不同方面EPF 1和EPF 2在调节气孔密度，涉及ER和TMM，但不SDD 1的途径是一致的。我们建议，EPF 2的表达早于EPF 1叶发育，并参与确定进入，并留在气孔谱系的细胞数量。
Stomata are pores in the plant epidermis that control carbon dioxide uptake and water loss. They are major regulators of global carbon and water cycles [1]. Several signaling components that regulate stomatal development have been characterized. These include a putative secretory peptide EPF1, LRR receptor components TMM and ER, and a peptidase SDD1 [2-4]. We have identified EPF2, a peptide related to EPF1 that is expressed in proliferating cells of the stomatal lineage, known as meristemoids, and in guard mother cells, the progenitors of stomata. EPF2 expression during leaf development affects stomatal density on the mature leaf. In the absence of EPF2, excessive numbers of cells enter the stomatal lineage and produce numerous small epidermal cells that express stomatal lineage reporter genes, whereas plants overexpressing EPF2 produce virtually no stomata. Results from genetic experiments indicate that EPF2 regulates a different aspect of stomatal development to EPF1 and are consistent with EPF2 acting in a pathway to regulate stomatal density that involves ER and TMM, but not SDD1. We propose that EPF2 is expressed earlier in leaf development than EPF1 and is involved in determining the number of cells that enter, and remain in, the stomatal lineage.