Development of a novel microfluidic device for long-term in situ monitoring of live cells in 3-dimensional matrices
Development of a novel microfluidic device for long-term in situ monitoring of live cells in 3-dimensional matrices
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DOI：
10.1007/s10544-012-9668-1
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发表时间：
2012-10-01
影响因子：
2.8
通讯作者：
Finne-Wistrand, Anna
中科院分区：
文献类型：
--
作者：
Danmark, Staffan;Gladnikoff, Micha;Finne-Wistrand, Anna
利用微制造技术的最新创新，三维微流控细胞培养系统已被开发为传统二维培养系统的有吸引力的替代方案，作为长期微尺度细胞研究的模型。大多数微流体系统是基于将细胞包埋在水凝胶中。然而，基于水凝胶的生理现实条件难以获得，并且系统通常过于复杂。我们已经开发了一种微流控细胞培养装置，该装置采用标准软光刻方法结合了可生物降解的刚性3D聚合物支架。该设备允许在4周的时间内对基质内的活细胞群体进行重复的高分辨率荧光成像。在这段时间内，也可以在同一空间位置跟踪细胞发育。此外，诱导人原代牙周膜细胞在系统内产生可定量的钙沉积。这种简单而多功能的设备应该很容易适用于需要长期培养和高分辨率生物成像的基于细胞的研究。
Using the latest innovations in microfabrication technology, 3-dimensional microfluidic cell culture systems have been developed as an attractive alternative to traditional 2-dimensional culturing systems as a model for long-term microscale cell-based research. Most microfluidic systems are based on the embedding of cells in hydrogels. However, physiologically realistic conditions based on hydrogels are difficult to obtain and the systems are often too complicated. We have developed a microfluidic cell culture device that incorporates a biodegradable rigid 3D polymer scaffold using standard soft lithography methods. The device permits repeated high-resolution fluorescent imaging of live cell populations within the matrix over a 4 week period. It was also possible to track cell development at the same spatial location throughout this time. In addition, human primary periodontal ligament cells were induced to produce quantifiable calcium deposits within the system. This simple and versatile device should be readily applicable for cell-based studies that require long-term culture and high-resolution bioimaging.