Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue
Immunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue
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DOI：
10.1136/jcp.54.9.684
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发表时间：
2001-09-01
影响因子：
3.4
通讯作者：
Kopolovic, J
中科院分区：
文献类型：
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作者：
Barshack, I;Goldberg, I;Kopolovic, J
背景-乳糜泻是一种慢性疾病，其中存在小肠的特征性粘膜病变和营养吸收受损，当从饮食中去除小麦麦醇溶蛋白和相关谷物蛋白后，情况会有所改善。活检标本显示弥漫性肠炎，伴有明显萎缩或绒毛完全丧失。还存在恶性疾病的长期风险。目的 - 比较乳糜泻儿童十二指肠粘膜中 DCC（结肠癌中缺失）、p53、E-钙粘蛋白和 β-连环蛋白的免疫表达与无小肠疾病证据的儿童的免疫表达。方法 - 为了更深入地了解乳糜泻儿童十二指肠上皮的遗传和免疫组织化学改变，对 21 例乳糜泻儿童的十二指肠上皮进行了内镜活检。对乳糜泻患者和无乳糜泻儿童的 10 份十二指肠活检组织进行免疫组织化学评估，以检测 p53、DCC、E-钙粘蛋白和 β-连环蛋白。 结果：与无乳糜泻患者相比，乳糜泻患者中 DCC 表达并未降低。 21 名乳糜泻患者中有 7 名出现 p53 阳性核免疫染色。阳性核染色主要见于隐窝的深部和侧面。对照组的所有患者p53均为阴性。在 21 名乳糜泻患者中，分别有 9 名和 3 名患者的 E-钙粘蛋白和 β-连环蛋白的免疫组织化学表达降低。而对照组的E-cadherin和β-catenin免疫染色均无变化。结论：与正常粘膜相比，乳糜泻患儿十二指肠上皮中E-cadherin和β-catenin均减少。 p53 在乳糜泻患者的十二指肠粘膜中过度表达。 E-钙粘蛋白和β-连环蛋白表达减少以及p53过度表达。可能导致乳糜泻小肠粘膜的形态变化。
Background-Coeliac sprue is a chronic disease, in which there is a characteristic mucosal lesion of the small intestine and impaired nutrient absorption, which improves upon the withdrawal of wheat gliadins and related grain proteins from the diet. Biopsy specimens demonstrate diffuse enteritis with pronounced atrophy or total loss of villi. There is also a long term risk of malignant disease.Aims-To compare the immunoexpression of DCC (deleted in colon cancer), p53, E-cadherin, and beta -catenin in the duodenal mucosa of children with coeliac disease with that seen in children with no evidence of small intestinal disease.Methods-To gain more insight into the genetic and immunohistochemical alterations of the duodenal epithelium in coeliac disease, 21 endoscopic biopsies from children with coeliac disease and 10 duodenal biopsies from children without coeliac disease were immunohistochemically evaluated for p53, DCC, E-cadherin, and beta -catenin.Results-DCC expression was not reduced in patients with coeliac disease compared with those without coeliac disease. p53 positive nuclear immunostaining was seen in seven of the 21 patients with coeliac disease. Positive nuclear staining was seen mainly in the deep and the lateral aspects of the crypts. All patients in the control group were negative for p53. In nine and three of the 21 patients with coeliac disease, respectively, the immunohistochemical expression of E-cadherin and beta -catenin was reduced. However, both E-cadherin and beta -catenin immunostaining in the control group was not altered.Conclusions-E-cadherin and beta -catenin were reduced in the duodenal epithelium of children with coeliac disease when compared with normal mucosa. p53 was overexpressed in the duodenal mucosa of patients with coeliac disease. The reduced expression of E-cadherin and beta -catenin and p53 overexpression. may contribute to the morphological changes seen in the small intestinal mucosa in coeliac disease.