Measurement of mucosal blood flow in the canine intestine with laser doppler velocimetry.
Measurement of mucosal blood flow in the canine intestine with laser doppler velocimetry.
复制标题
用激光多普勒测速仪测量犬肠道粘膜血流量。
DOI：
10.1016/0024-3205(82)90013-3
复制
发表时间：
1982
期刊：
影响因子：
6.1
通讯作者：
Jacobson,ED
中科院分区：
文献类型：
--
作者：
Feld,AD;Fondacaro,JD;HollowayJr,GA;Jacobson,ED
激光多普勒测速是一种用于连续估计组织表面中变化的血流的技术，并且不需要侵入循环。该技术基于多普勒原理，即从移动物体发射或反射的电磁波的频率偏移与物体的速度成比例。在犬自由流准备中测试激光多普勒测速仪估计肠粘膜血流变化的能力。在麻醉的狗，其中一段回肠被隔离，同时测量的瞬时变化的总血流量（测量与电磁血流计）和瞬时变化的假定粘膜血流（使用激光多普勒测速仪）。在休息、前列环素诱导的血管舒张和去甲肾上腺素诱导的血管收缩条件下进行测定。激光多普勒测速仪读数的变化定性相似，并在时间上与血管活性药物给药期间肠段总血流量的变化相关。变化的幅度和方向与两个测量值显著相关。将激光探针稳定在粘膜表面以确保可重复的读数被证明是技术上困难的。药理学诱导的变化，激光多普勒测速仪估计的流量变化更容易与电磁流量计读数的变化比对照值获得的两种方法。
Laser Doppler velocimetry is a technique for continuous estimation of changing blood flow in the surface of a tissue and does not require invasion of the circulation. This technique is based upon the Doppler principle that a shift in the frequency of an electromagnetic wave emitted or reflected from a moving object is proportional to the velocity of the object. The capacity of Laser Doppler velocimetry to estimate changes in intestinal mucosal blood flow was tested in a canine free flow preparation. In anesthetized dogs in which a segment of ileum was isolated, simultaneous measurements of instantaneous changes in total blood flow (measured with the electromagnetic blood flow meter) and instantaneous changes in presumed mucosal blood flow (using laser Doppler velocimetry) were obtained. Determinations were made during conditions of rest, prostacyclin induced vasodilation and norepinephrine induced vasoconstriction. Changes in laser Doppler velocimeter readings were qualitatively similar to and temporally related to changes in total blood flow to the gut segment during administration of the vasoactive drugs. The magnitude and direction of changes with the two measurements were significantly correlated. Stabilizing the laser probe on the mucosal surface to ensure reproducible readings proved technically difficult. Pharmacologically induced changes in laser Doppler velocimeter estimated changes in flow were more readily correlated with changes in electromagnetic flow meter readings than were control values obtained with the two methods.