Expression, genetic localization and phylogenic analysis of NAPlr in piscine Streptococcus dysgalactiae subspecies dysgalactiae isolates and their patterns of adherence
Expression, genetic localization and phylogenic analysis of NAPlr in piscine Streptococcus dysgalactiae subspecies dysgalactiae isolates and their patterns of adherence
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DOI：
10.1016/j.jare.2014.05.005
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发表时间：
2015-09-01
影响因子：
10.7
通讯作者：
Warda, M.
中科院分区：
文献类型：
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作者：
Abdelsalam, M.;Fujino, M.;Warda, M.
乳链球菌是一种公认的哺乳动物病原体，目前已经引起了人们对鱼类细菌感染的关注。与宿主上皮细胞的黏附和壁上相关纤溶酶原结合蛋白的存在是链球菌感染的先决条件。这是首次研究肾炎相关纤溶酶原结合受体(NAPlr)和α-烯醇酶基因在鱼类异常乳链球菌亚种异常(SDSD)分离株中的出现情况。进一步对鱼SDSD表面定位的NAPlR进行了鉴定，发现了一条43 KDa的免疫反应条带，与猪链霉菌亚种相似。等同(SDSE)。系统发育分析表明，鱼类SDSD的NAPlR与哺乳动物SDSE和化脓性链球菌的NAPlR同源性较高，而与其他链球菌的同源性较低。我们的发现值得公众关注这些毒力基因在SDSD传播到受感染宿主的不同组织中的可能含义，并获得新的利基。为了更好地了解它们的致病性，还研究了SDSD的黏附模式。SDSD在两种不同细胞株上的黏附模式不同。这种差异使我们对宿主感染易感性的差异有了更深入的了解。(C)2014年由Elsevier B.V.代表开罗大学制作和主办。
Streptococcus dysgalactiae, the long recognized mammalian pathogen, has currently received a major concern regarding fish bacterial infection. Adhesion to host epithelial cells and the presence of wall-associated plasminogen binding proteins are prerequisites to Streptococcus infection. This is the first study of the occurrence of nephritis-associated plasminogen-binding receptor (NAPlr) and alpha-enolase genes in piscine S. dysgalactiae subspecies dysgalactiae (SDSD) isolates. Further characterization of surface localized NAPlr of fish SDSD revealed a similar immune-reactive band of 43 KDa as that from porcine S. dysgalactiae subsp. equisimilis (SDSE). The phylogenetic analysis revealed that NAPlr of fish SDSD is more associated with those of mammalian SDSE and Streptococcus pyogenes rather than of other streptococci. Our findings warrant public attention to the possible implication of these virulence genes in dissemination of SDSD to different tissues of infected hosts and to get advantage to new niches. The SDSD adherence patterns were also studied to better understand their pathogenicity. The patterns of adherence of SDSD on two different cell lines showed a different pattern of adherence. Such difference gives an insight about the variance in host susceptibility to infection. (C) 2014 Production and hosting by Elsevier B.V. on behalf of Cairo University.