Nondestructive and continuous observation of nectar volume using time-interval photographhy.

Nondestructive and continuous observation of nectar volume using time-interval photographhy.
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使用时间间隔摄影对花蜜量进行无损连续观察。

DOI:
10.1111/1442-1984.12007
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发表时间:
2013
影响因子:
1.4
通讯作者:
N.
N.
中科院分区:
生物学4区
文献类型:
--
作者:
Jahir;R. and Kawakubo;N.

文献摘要

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为了观察凤仙花花蜜量的时间变化,我们修改并使用了带有特殊闪光系统的间隔拍摄相机。以前测量花蜜量的方法不可避免地需要破坏或损坏花的部分。因此,在自然条件下精确连续测量花蜜量一直是困难的。在考虑如何克服这个问题时,我们注意到,当在太阳透射光下观察花朵时,可以在花距内看到花蜜的轮廓。为了利用这种现象,我们将一根聚合物光纤连接到紧凑型数码相机的内置闪光灯上,并将光纤向相机的透镜弯曲,以提供背光。我们拍摄了间隔图像,并从花蜜轮廓的大小估计了花蜜体积。据我们所知,这是第一个报道的方法,涉及使用时间间隔摄影测量花蜜体积原位。
To observe temporal changes in the nectar volume ofImpatiensflowers, we modified and used an interval‐shooting camera with a special flash system. Former methods of measuring nectar volume inevitably necessitated destruction or damage of the floral parts. As a consequence, accurate continuous measurements of nectar volume under natural conditions have been difficult. While considering how to overcome this problem we noticed that when flowers were observed against transmitted light from the sun, a silhouette of nectar was visible inside the spur. To exploit this phenomenon, we attached a polymer optical fiber to the built‐in flash of a compact digital camera and bent the fiber towards the camera's lens to provide backlighting. We took interval images and estimated the nectar volume from the size of nectar silhouette. To our knowledge, this is the first reported method involving the use of time‐interval photography for measuring nectar volumein situ.