No change in neuron numbers in the dentate nucleus of patients with schizophrenia estimated with a new stereological method - the smooth fractionator
No change in neuron numbers in the dentate nucleus of patients with schizophrenia estimated with a new stereological method - the smooth fractionator
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DOI：
10.1111/j.0021-8782.2004.00337.x
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发表时间：
2004-10-01
影响因子：
2.4
通讯作者：
Stark, AK
中科院分区：
文献类型：
--
作者：
Andersen, BB;Fabricius, K;Stark, AK
齿状核是小脑发育最晚的核团。由于它与丘脑和前额叶皮层的联系，它可能与精神分裂症和其他精神疾病的病理学有关。在这项体视学研究中，我们实现了平滑分馏器，它结合了光学分馏器的无偏原则，一个新的和更有效的采样策略的齿状核。平滑分馏器代表了体视学中迄今为止描述的最有效的采样策略，其降低了采样方差，从而提高了效率。这是第一个应用程序的顺利分馏人脑组织，并提出了估计的神经元总数在齿状核的8例精神分裂症患者相比，8个控制人。在精神分裂症患者中，齿状核中的神经元总数估计为3.36 × 10（6），与对照组的3.65 × 10（6）相比，没有统计学显著差异（P = 0.63）。讨论了光滑分馏塔法的优缺点，并对其在实际应用中的精度进行了估计。
The dentate nucleus is phylogenetically the most recent nucleus in the cerebellum. Owing to its connections to the thalamus and the prefrontal cortex it may be involved in the symptomathology in schizophrenia and other psychiatric illnesses. In this stereological study we implemented the smooth fractionator, which combines the unbiased principles of the optical fractionator with a new and more efficient sampling strategy to the dentate nucleus. The smooth fractionator represents the most efficient sampling strategy described so far in stereology, in terms of reducing the sampling variance and thus increasing the efficiency. It is the first application of the smooth fractionator to human brain tissue and presents estimations of total number of neurons in the dentate nuclei of eight patients with schizophrenia compared to eight control persons. The total number of neurons in the dentate nucleus was estimated to 3.36 x 10(6) in subjects with schizophrenia, which was not statistically significant different from 3.65 x 10(6) in control subjects (P = 0.63). The advantages and disadvantages of the smooth fractionator method are discussed and its precision in practical application is estimated.