Arabidopsis Plants Overexpressing the MsDREB2C Exhibit Increased Susceptibility to Alternaria mali Infection
Arabidopsis Plants Overexpressing the MsDREB2C Exhibit Increased Susceptibility to Alternaria mali Infection
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DOI：
10.1007/s00344-014-9444-y
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发表时间：
2015-03
影响因子：
4.8
通讯作者：
K. Zhao;Xinwei Guo;Xiong Liao;Qi Wang;Dong Liu;Tianhong Li
中科院分区：
文献类型：
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作者：
K. Zhao;Xinwei Guo;Xiong Liao;Qi Wang;Dong Liu;Tianhong Li
脱水响应元件结合 (DREB) 通过与各种非生物胁迫响应基因启动子区域中存在的 DRE/CRTcis 元件相互作用，在提高植物的胁迫耐受性方面发挥着关键作用。本研究从海棠中分离和鉴定了一个DREB2同源基因，命名为MsDREB2C（GenBank登录号JQ790526）。 MsDREB2C 定位于拟南芥原生质体的细胞核。 qRT-PCR分析表明MsDREB2C是由水杨酸(SA)和茉莉酸(JA)诱导的。此外，过表达MsDREB2C的转基因拟南芥植物对黑斑病感染的敏感性增加，并且转基因植物中MsDREB2C的组成型表达在正常和致病条件下抑制PR基因的表达和过氧化物酶的活性。然后我们对 MsDREB2C 启动子区域进行功能表征。删除分析确定了负责基因对 SA（-335 至 -148 bp）和 JA（-831 至 -680 bp 和 -335 至 -148 bp）反应的调控区域。这些结果表明MsDREB2C的过度表达降低了对A.mali的抗性。
Dehydration responsive element binding (DREBs) play a critical role in improving the stress tolerance of plants by interacting with a DRE/CRTcis-element present in the promoter region of various abiotic stress-responsive genes. In this study, aDREB2homologous gene fromMalus sieversiiRoem., designatedMsDREB2C(GenBank accession No. JQ790526), was isolated and characterized. MsDREB2C was localized to the nucleus ofArabidopsisprotoplasts. qRT-PCR analysis demonstrated thatMsDREB2Cwas induced by salicylic acid (SA) and jasmonic acid (JA). Furthermore, transgenicArabidopsisplants overexpressingMsDREB2Cexhibited increased sensitivity toAlternaria maliinfection, and the constitutive expression ofMsDREB2Cin transgenic plants repressed the expression ofPRgenes and the activity of peroxidase under normal and pathogenic conditions. We then functionally characterized theMsDREB2Cpromoter region. Deletion analysis identified the regulatory regions responsible for the gene’s response to SA (−335 to −148 bp) and JA (−831 to −680 bp and −335 to −148 bp). These results suggested that overexpression ofMsDREB2Cdecreased resistance toA.mali.