Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops
Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops
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DOI：
10.1038/s41594-022-00780-0
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发表时间：
2022-06-16
影响因子：
16.8
通讯作者：
Koszul, Romain
中科院分区：
文献类型：
--
作者：
Bastie, Nathalie;Chapard, Christophe;Koszul, Romain
内聚蛋白是一种DNA转位酶，在基因组折叠成染色质环的过程中起着重要作用，对DNA相关过程具有功能影响。染色质环的长度和组织可能取决于内聚性、易位率和DNA上的稳定性。在这里，我们调查并提供了一个全面的概述，各种粘接蛋白调节调节染色质环扩大在出芽酵母酿酒酵母的作用。我们证明，Scc2，刺激黏结蛋白atp酶活性，也是黏结蛋白易位，驱动环扩张在体内的必要条件。S期的Smc3乙酰化通过稳定Pds5抵消了这种活性，Pds5精细地调节了G2中环的大小和稳定性。
Cohesin is a DNA translocase that is instrumental in the folding of the genome into chromatin loops, with functional consequences on DNA-related processes. Chromatin loop length and organization likely depend on cohesin processivity, translocation rate and stability on DNA. Here, we investigate and provide a comprehensive overview of the roles of various cohesin regulators in tuning chromatin loop expansion in budding yeast Saccharomyces cerevisiae. We demonstrate that Scc2, which stimulates cohesin ATPase activity, is also essential for cohesin translocation, driving loop expansion in vivo. Smc3 acetylation during the S phase counteracts this activity through the stabilization of Pds5, which finely tunes the size and stability of loops in G2.