A New Animal Model to Investigate Axonal Sprouting After End-to-Side Neurorrhaphy
A New Animal Model to Investigate Axonal Sprouting After End-to-Side Neurorrhaphy
复制标题
一种研究端侧神经缝合术后轴突萌芽的新动物模型
DOI：
10.1055/s-2007-1006410
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发表时间：
1997
影响因子：
2.1
通讯作者：
J. Terzis
中科院分区：
文献类型：
--
作者：
E. Noah;Armistead D. Williams;Williefred Fortes;J. Terzis
端侧神经缝合术是一种可以在不损伤原始供体神经的情况下解决远端目标神经支配问题的技术。 1992年维泰博报道了他的实验后，这项技术引起了广泛的关注；然而，迄今为止，用于阐明侧轴突萌芽过程的动物模型存在对供体神经造成严重损伤的缺点，引发了关于重新支配神经移植物的轴突起源的问题。在本报告中，引入了一种新的大鼠模型，其中供体神经未受损，并且可以通过神经移植物支配额外的目标。隐神经代表轴突导管；近端在神经周围窗的位置处端对侧地接合到坐骨神经。远端穿过内收肌并以端对端的方式与闭孔神经向远端接合。在一组中，在接合部位进行了部分神经切除术，这导致坐骨功能指数（SFI）降低。在第二组中，神经周围窗的创建在端侧神经缝合术后产生了正常的 SFI。与部分神经切除组相比，神经周围窗端侧神经缝合术导致移植物中的轴突明显减少。新模型具有以下优点：（a）对供体神经的损伤最小； (b) 提供一个单一的额外目标（gracilis），其功能恢复可以从形态和行为上进行评估； (c) 通过提供非损伤模型来了解侧芽，其中移植物的轴突侵入可能源自结节轴突生长； (d)建立可广泛临床应用的非损伤模型。
End-to-side neurorrhaphy is a technique that may provide a solution for the problem of distal target reinnervation without injury to the original donor nerve. The technique drew extensive attention after Viterbo reported his experiments in 1992; however, to date, the animal models used to elucidate the process of lateral axon sprouting had the disadvantage of substantial injury to the donor nerve, raising questions about the origin of axons reinnervating the nerve graft. In this report, a new model in the rat is introduced, in which the donor nerve is not damaged and an additional target can be innervated via a nerve graft. The saphenous nerve represents the axonal conduit; the proximal end is coapted end-to-side to the sciatic nerve at the site of a perineurial window. The distal end is passed through the adductor muscles and coapted distally in an end-to-end fashion with the obturator nerve. In one group, a partial neurectomy was performed at the site of coaptation, which led to a lower Sciatic Functional Index (SFI). In the second group, the creation of a perineurial window yielded a normal SFI after end-to-side neurorrhaphy. Compared to the partial neurectomy group, the perineurial window end-to-side neurorrhaphy resulted in significantly less axons in the graft. The new model has the following advantages: (a) minimal injury to the donor nerve; (b) provision of a single additional target (gracilis) whose functional recovery can be assessed morphologically and behaviorally; (c) an opportunity to understand lateral sprouting by providing a non-injury model in which axonal invasion of the graft can originate from nodal axonal outgrowth; and (d) establishment of a noninjury model that can have widespread clinical applications.