Enhanced expression of uterine stathmin during the process of implantation and decidualization in rats.
Enhanced expression of uterine stathmin during the process of implantation and decidualization in rats.
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DOI：
10.1210/en.2002-220834
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发表时间：
2003-04
期刊：
影响因子：
4.8
通讯作者：
K. Tamura;T. Hara;M. Yoshie;Shinya Irie;A. Sobel;H. Kogo
中科院分区：
文献类型：
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作者：
K. Tamura;T. Hara;M. Yoshie;Shinya Irie;A. Sobel;H. Kogo
我们使用文库消减技术来鉴定在妊娠早期大鼠子宫中特异表达的基因。一个这样的基因是stathmin，一个与微管蛋白结合和微管不稳定相关的因子。Stathmin在妊娠第6天和第7天着床部位的表达水平高于着床部位;妊娠第6天和第7天着床部位的表达水平高于妊娠第3-5天整个子宫或非妊娠子宫。stathmin mRNA的强烈表达主要限于植入部位的腔下基质细胞。在蜕膜区也检测到表达，并在蜕膜化期间（d 7-12）加重。在延迟着床妊娠大鼠模型中，子宫stathmin的表达较低，但在给药17 β-雌二醇诱导的着床后增加。此外，子宫内注入油诱导的假孕大鼠蜕膜化增强了stathmin的表达。Stathmin的表达明显增加，在子宫内胚胎植入和蜕膜化刺激时，可能在妊娠早期发挥作用。
We used the library subtraction technique to identify genes specifically expressed in the rat uterus during early pregnancy. One such gene was that for stathmin, a factor that is associated with tubulin binding and the destabilization of microtubules. Stathmin was expressed at higher levels in implantation sites than in interimplantation sites on d 6 and 7 of pregnancy; the levels on d 6 and 7 were higher in implantation sites than in the entire uterus on d 3-5 of pregnancy or in nonpregnant uteri. Intense expression of stathmin mRNA was primarily limited to the subluminal stromal cells at the implantation site. Expression was also detected in the decidual zones and was accentuated during the period of decidualization (d 7-12). In the delayed implantation pregnant rat model, uterine stathmin expression was low, but increased after implantation induced by administration of 17beta-estradiol to the progesterone-primed animal. Further, decidualization in the pseudopregnant rat, induced by intrauterine infusion of oil, enhanced stathmin expression. Stathmin expression clearly increases in the uterus when stimulated by embryo implantation and decidualization and may play a role in the early stages of pregnancy.