Rapid and Sensitive Analysis of Volatile Components of Different Parts of Clausena lansium by Ionic Liquid Based Headspace Gas Chromatography-Mass Spectrometry
Rapid and Sensitive Analysis of Volatile Components of Different Parts of Clausena lansium by Ionic Liquid Based Headspace Gas Chromatography-Mass Spectrometry
复制标题
离子液体顶空气相色谱-质谱法快速灵敏分析黄皮不同部位的挥发性成分
DOI：
10.3390/molecules24010091
复制
发表时间：
2019-01-01
期刊：
影响因子：
4.6
通讯作者：
Zhang, Lantong
中科院分区：
文献类型：
--
作者：
He, Xiaowen;Ma, Yinzheng;Zhang, Lantong
建立了一种基于离子液体(IL)的顶空气相色谱质谱(HS-GC-MS)快速、灵敏的分析海南不同产地黄皮、果皮和种子中挥发性成分的方法，中国。以三种离子液体和水为基质介质，仔细研究了HS的效率。采用OA(9)(3(3))表进行正交设计，对平衡温度、平衡时间、搅拌速度等提取工艺参数进行了优化。在基于离子液体的HS-GC-MS优化条件下，只需100 mg样品和2毫升[Bmim][BF4]即可全面、准确地分析黄皮中的挥发性成分。通过聚类分析，得到了90个组分的6个聚类。与以前报道的分析和鉴定黄皮挥发性成分的方法相比，该方法更简单、更快速、更灵敏。研究结果可为黄皮的进一步开发利用提供理论依据。
A rapid and sensitive ionic liquid (IL) based headspace gas chromatography-mass spectrometry (HS-GC-MS) method was developed for analyzing volatile components in leaf, pericarp, and seed of Clausena lansium from different areas in Hainan Province, China. HS efficiencies were carefully investigated by using three ILs and water as matrix media. Extraction parameters, including equilibrium temperature, equilibrium time, and stirring rate had been evaluated and optimized by using an orthogonal design with OA(9)(3(3)) table. Under the optimized condition of IL-based HS-GC-MS, only 100 mg of sample and 2 mL of [Bmim][BF4] were needed to comprehensively and accurately analyze the volatile components in Clausena lansium. By utilizing a cluster analysis, six clusters were obtained for ninety components. This method was simpler, more rapid, and more sensitive when compared with previously reported methods for analyzing and identifying volatile components in Clausena lansium. The results may provide a theoretical basis for further exploitation of Clausena lansium.