A simple and rapid HPLC method for the detection of glyburide in plasma original research communication (analytical)
A simple and rapid HPLC method for the detection of glyburide in plasma original research communication (analytical)
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DOI：
10.1016/j.clinbiochem.2007.07.025
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发表时间：
2008-02-01
影响因子：
2.8
通讯作者：
Koren, Gideon
中科院分区：
文献类型：
--
作者：
Gedeon, Christelle;Kapur, Bhushan;Koren, Gideon
简介：尽管之前已经描述了用于格列本脲定量的各种HPLC方法，但临床上实施这些方法的尝试并不成功。一些是耗时的、昂贵的，或不直接适用于人血浆。目的：建立一种简便、快速、灵敏的高效液相色谱法（HPLC），用于人体血浆和灌流液的分析。方法：样品经氯仿液-液萃取，在254 nm波长处检测，每个样品的总运行时间为7 min。结果：格列本脲的标准曲线在50 - 400 ng/mL范围内呈线性（r（2）=0.998）。日间和日内样品变异系数分别为8%和4%。在20-400 ng/mL.Conclusion：本法可应用于临床格列本脲的治疗药物监测。(c)2007年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Introduction: Although a variety of HPLC methods have been previously described for the quantification of glyburide, attempts to implement them clinically have been unsuccessful. Some are time consuming, expensive, or not directly applicable to human plasma. Others are outdated, as the necessary materials are no longer available.Objective: To describe a simple, rapid, and sensitive HPLC method for the analysis of human plasma and perfusate.Design and method: Samples were extracted by liquid-liquid extraction with chloroform at neutral pH. Glyburide was detected at 254 nm, with a total run time of 7 min per sample.Results: Standard curves of 50 to 400 ng/mL of glyburide were linear (r(2)=0.998). Inter-day and infra-day sample coefficient of variations were 8% and 4%, respectively. Recoveries ranged from 71 to 75% in human plasma samples for the 20-400 ng/mL concentration range.Conclusion: Clinically, this method can be applied in the therapeutic drug monitoring of glyburide. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.