COMPLETE NUCLEOTIDE-SEQUENCE OF THE ESCHERICHIA-COLI RECB GENE
COMPLETE NUCLEOTIDE-SEQUENCE OF THE ESCHERICHIA-COLI RECB GENE
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DOI：
10.1093/nar/14.21.8573
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发表时间：
1986-11-11
影响因子：
14.9
通讯作者：
EMMERSON, PT
中科院分区：
文献类型：
--
作者：
FINCH, PW;STOREY, A;EMMERSON, PT
已确定编码ATP依赖性DNA酶（核酸外切酶V）亚基的土方ichiacolirecB基因的完整核苷酸序列。RecB蛋白的拟议编码区长3543个核苷酸，编码1180个氨基酸的多肽，计算分子量为133，973。recB的起始密码子与上游ptr基因的3′端重叠，recB的终止密码子与下游recD基因的起始密码子重叠，提示这些基因可能形成一个操纵子。没有合理符合E共有序列的序列。大肠杆菌启动子可以确定上游的建议recB翻译开始。预测的RecBa氨基酸序列含有与ATP酶、DNA结合蛋白和DNA修复酶同源的区域。
The complete nucleotide sequence of theEscherichiacolirecBgene which encodes a subunit of the ATP-dependent DNase, Exonuclease V, has been determined. The proposed coding region for the RecB protein is 3543 nucleotides long and would encode a polypeptide of 1180 ami no acids with a calculated molecular weight of 133,973. The start of therecBcoding sequenoe overlaps the 3′ end of the upstream ptr gene, and therecBtermination codon overlaps the initiation codon of the downstreamrecDgene, suggesting that these genes may form an operon. No sequences which reasonably fit the consensus for anE. colipromoter could be identified upstream of the proposed recB translational start. The predictedRecBamino aoid sequence contains regions of homology with ATPases, DNA binding proteins and DNA repair enzymes.