The mRNA decapping protein 2 (DCP2) is a major regulator of developmental events in Drosophila-insights from expression paradigms
The mRNA decapping protein 2 (DCP2) is a major regulator of developmental events in Drosophila-insights from expression paradigms
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DOI：
10.1007/s00441-021-03503-x
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发表时间：
2021-09-18
影响因子：
3.6
通讯作者：
Roy, Jagat Kumar
中科院分区：
文献类型：
--
作者：
Kunar, Rohit;Roy, Jagat Kumar
果蝇基因组编码两种脱帽蛋白，DCP1和DCP2，其中DCP2是活性脱帽酶。本研究探索了DCP2在果蝇体内的内源性启动子激活、转录和蛋白表达，其中，除了在整个发育过程中普遍存在的表达外，我们还在幼虫大脑的Corazonin神经元中发现了一种活跃的表达范式，以及一种可能的月光表达。我们还证明，DCP2的缺失会导致胚胎死亡和重要形态发生过程的缺陷，而Corazonin神经元中DCP2的缺失会降低成人对乙醇的敏感性，从而将DCP2归因于新的调节作用。我们的研究结果揭示了DCP2的新假设作用，并将其确定为果蝇和生物世界早期发育过程中基本分子调节相互作用的研究候选者。
The Drosophila genome codes for two decapping proteins, DCP1 and DCP2, out of which DCP2 is the active decapping enzyme. The present endeavour explores the endogenous promoter firing, transcript and protein expression of DCP2 in Drosophila wherein, besides a ubiquitous expression across development, we identify an active expression paradigm during dorsal closure and a plausible moonlighting expression in the Corazonin neurons of the larval brain. We also demonstrate that the ablation of DCP2 leads to embryonic lethality and defects in vital morphogenetic processes whereas a knockdown of DCP2 in the Corazonin neurons reduces the sensitivity to ethanol in adults, thereby ascribing novel regulatory roles to DCP2. Our findings unravel novel putative roles for DCP2 and identify it as a candidate for studies on the regulated interplay of essential molecules during early development in Drosophila, nay the living world.