κ-carrageenan induces the disruption of intestinal epithelial Caco-2 monolayers by promoting the interaction between intestinal epithelial cells and immune cells
κ-carrageenan induces the disruption of intestinal epithelial Caco-2 monolayers by promoting the interaction between intestinal epithelial cells and immune cells
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DOI：
10.3892/mmr.2013.1726
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发表时间：
2013-12-01
影响因子：
3.4
通讯作者：
Yan, Xiao-Jun
中科院分区：
文献类型：
--
作者：
Jiang, Hai-Yue;Wang, Feng;Yan, Xiao-Jun
- 角叉菜胶（-CGN）是一种重要的食品添加剂，已被证明在动物模型中诱导结肠炎。在本研究中，使用体外共培养系统，其中包含肠上皮Caco-2细胞和活化的巨噬细胞样THP-1细胞的影响进行了评估-CGN。结果表明，在单一培养的Caco-2和THP-1细胞中，-CGN处理的细胞毒性和IL-1，IL-6和TNF-α的分泌水平较低。然而，在共培养系统中，-CGN处理导致细胞凋亡，并降低了Caco-2细胞单层的跨上皮电阻。两种细胞分泌TNF-α、IL-1和IL-6的水平均显著增加.此外，预处理的共培养系统与抗TNF受体1抗体抑制CGN诱导的细胞凋亡和衰减的Caco-2单层中的IL-6和IL-1的水平的变化。这项研究表明，CGN诱导的TNF-α分泌是暴露于CGN的Caco-2单层细胞损伤的主要贡献者。
-carrageenan (-CGN) is an important food additive that has been demonstrated to induce colitis in animal models. In the present study, the effects of -CGN were assessed using an in vitro co-culture system that contained intestinal epithelial Caco-2 cells and activated macrophage-like THP-1 cells. The results demonstrated that in single cultures of Caco-2 and THP-1 cells treated with -CGN, the cytotoxicity and the secretion levels of IL-1, IL-6 and TNF- were low. In the co-culture system, however, -CGN treatment resulted in apoptosis and reduced the transepithelial electrical resistance of the Caco-2 cell monolayers. The secretion levels of TNF-, IL-1 and IL-6 from the two cell types were increased significantly by -CGN treatment. Furthermore, pretreatment of the co-culture system with anti-TNF receptor 1 antibody suppressed the -CGN-induced apoptosis and attenuated the changes in the levels of IL-6 and IL-1 in the Caco-2 monolayers. This study indicated that -CGN-induced TNF- secretion is the main contributor to cellular damage in Caco-2 monolayers exposed to -CGN.