Tomato SlIDA has a critical role in tomato fertilization by modifying reactive oxygen species homeostasis
Tomato SlIDA has a critical role in tomato fertilization by modifying reactive oxygen species homeostasis
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番茄 SlIDA 通过改变活性氧稳态在番茄受精中发挥关键作用
DOI：
10.1111/tpj.14886
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发表时间：
2020
期刊：
影响因子：
7.2
通讯作者：
Li Tianlai
中科院分区：
文献类型：
--
作者：
Wang Rong;Shi ChunLin;Wang Xiaoyang;Li Ruizhen;Meng Yan;Cheng Lina;Qi Mingfang;Xu Tao;Li Tianlai
花药发育和花粉管伸长是授粉和受精的关键步骤。活性氧（ROS）和程序性细胞死亡的时间和空间分布是这些过程的核心，但ROS产生的调控机制还没有很好地理解。花序缺乏分化（IDA）与许多植物的发育和对环境刺激的反应有关。然而，它们在生殖发育中的作用仍然未知。我们产生了IDA同源物（SlIDA）的番茄敲除系（CR‐slida），该同源物在绒毡层、隔膜和花粉管中表达，并观察到雄性配子的严重缺陷。进一步的分析表明，绒毡层和隔膜中存在程序性细胞死亡缺陷，CR-slideline中花药开裂失败，可能与ROS信号不足有关。液相色谱-串联质谱法鉴定成熟的SlIDA为14-mer EPIP肽，其被证明是分泌的，并且互补实验表明，合成的14-mer EPIP肽的应用挽救了CR-slida缺陷并增强了ROS信号。此外，ROS清除剂二亚苯基碘鎓或Mn-TMPP的应用抑制了肽功能。总之，我们的研究结果表明，SlIDA通过调节ROS的稳态在花粉发育和花粉管伸长中起着至关重要的作用。
Anther development and pollen tube elongation are key steps for pollination and fertilization. The timing and spatial distribution of reactive oxygen species (ROS) and programmed cell death are central to these processes, but the regulatory mechanism of ROS production is not well understood. Inflorescence deficient in abscission (IDA) is implicated in many plant development and responses to environmental stimuli. However, their role in reproductive development is still unknown. We generated tomato knockout lines (CR‐slida) of an IDA homolog (SlIDA), which is expressed in the tapetum, septum and pollen tube, and observed a severe defect in male gametes. Further analysis indicated that there was a programmed cell death defect in the tapetum and septum and a failure of anther dehiscence in theCR‐slidalines, likely related to insufficient ROS signal. Liquid chromatography‐tandem mass spectrometry identified mature SlIDA as a 14‐mer EPIP peptide, which was shown to be secreted, and a complementation experiment showed that application of a synthetic 14‐mer EPIP peptide rescued theCR‐slidadefect and enhanced the ROS signal. Moreover, the application of the ROS scavengers diphenyleneiodonium or Mn‐TMPP suppressed peptide function. Collectively, our results revealed thatSlIDAplays an essential role in pollen development and pollen tube elongation by modulating ROS homeostasis.