Porcine Reproductive and Respiratory Syndrome Virus isolates differ in their susceptibility to neutralization
Porcine Reproductive and Respiratory Syndrome Virus isolates differ in their susceptibility to neutralization
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DOI：
10.1016/j.vaccine.2011.07.076
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发表时间：
2011-09-16
期刊：
影响因子：
5.5
通讯作者：
Prieto, Cinta
中科院分区：
文献类型：
--
作者：
Javier Martinez-Lobo, F.;Diez-Fuertes, Francisco;Prieto, Cinta
猪繁殖与呼吸综合征病毒(PRRSV)具有高度异质性。这种异质性对PRRSV的抗原组成有影响，并可能造成不同分离株对中和敏感性的差异。对中和的敏感性可能是PRRSV分离株的一个重要特征，因为对中和耐药的分离株可能对开发引起广泛保护性免疫的疫苗构成重大挑战。尽管如此，了解或分类PRRSV分离株的病毒中和表型的信息很少。因此，本研究的主要目的是确定PRRSV分离株对中和的敏感性是否存在差异，以及是否可以根据其中和表型将其划分为不同的类别。为此，一组39个PRRSV分离株和一组30个高免单特异性血清被用于交叉中和试验。本研究的结果表明，PRRSV分离株对中和的敏感性不同，K-Means聚类系统允许根据其中和表型将分离株分为四类：高度敏感、敏感、中等敏感和抵抗中和。使用另外两个考虑个别数据对分离物进行分类的聚类系统的进一步分析证实，k-Means分类在大多数情况下是准确的，只有在少数情况下分类不那么严格。Gp3基因序列。对GP4和GP5进行了分析，但没有发现先前确定的中和表位序列或不同蛋白中N连接的糖基化位点数量与分离株的中和表型之间存在相关性。这些数据首次对一组不同的PRRSV分离株的总体中和敏感性进行了系统评估。对分离株的分类提供了一个有用的工具，有助于系统地表征由新的候选疫苗引起的中和抗体的产生。(C)2011爱思唯尔有限公司。保留所有权利。
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is highly heterogenic. This heterogeneity has an effect on antigenic composition of PRRSV and might create differences in sensitivity to neutralization between isolates. The sensitivity to neutralization could be an important feature of PRRSV isolates because it is likely that isolates resistant to neutralization pose a significant challenge for the development of vaccines that elicit broad protective immunity. Nonetheless, little information is available for understanding or categorizing the viral neutralization phenotype of PRRSV isolates. Consequently, the main purpose of this study was to determine whether PRRSV isolates differ in their susceptibility to neutralization and if they can be classified in different categories based on their neutralization phenotype. For this purpose, a panel of 39 PRRSV isolates and a set of 30 hyperimmune monospecific sera were used in cross-neutralization assays. The results of this study indicate that PRRSV isolates differ in their sensitivity to neutralization and k-means clustering system allowed classifying the isolates in four different categories according to their neutralization phenotype: highly sensitive, sensitive, moderately sensitive and resistant to neutralization. Further analyses using two additional clustering systems that considered individual data for the classification of the isolates confirmed that classification obtained by k-means is accurate in most cases and that only in a few instances classification is less stringent. Sequences of GP3. GP4 and GP5 were analyzed but no correlation could be found between the sequence of previously identified neutralizing epitopes or the number of N-linked glycosylation sites in different proteins and the neutralization phenotype of the isolates. These data provide the first systematic assessment of overall neutralization sensitivities of a panel of diverse PRRSV isolates. The classification of the isolates provides a useful tool to facilitate the systematic characterization of neutralizing antibody production elicited by new vaccine candidates. (C) 2011 Elsevier Ltd. All rights reserved.