Effects of atrial natriuretic factor and vasopressin on cyclic nucleotides in cultured kidney cells.
Effects of atrial natriuretic factor and vasopressin on cyclic nucleotides in cultured kidney cells.
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心房钠尿因子和加压素对培养肾细胞中环核苷酸的影响。
DOI：
10.1161/01.hyp.11.4.392
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Fejes-Tóth,G
中科院分区：
文献类型：
--
作者：
Náray-Fejes-Tóth,A;Carretero,OA;Fejes-Tóth,G
心钠素（ANF）作用的细胞机制被认为涉及鸟苷酸环化酶的激活。越来越多的证据表明ANF具有直接的肾小管效应。部分ANF诱导的利尿作用被认为是由于抑制皮质集合小管中精氨酸加压素（AVP）的作用。在这项研究中，我们研究了心钠素对皮质集合小管细胞免疫单克隆抗体免疫原代培养的环核苷酸生产的影响。ANF引起环鸟苷3 '，5'-单磷酸（cGMP）产生的剂量依赖性刺激;在约1 nM ANF时观察到半最大刺激。ANF（0.01-100 nM）对皮质集合小管培养物中环腺苷3 '，5'-单磷酸（cAMP）的积累没有影响。AVP引起cAMP产生的剂量依赖性增加，并且这种效应不被同时添加ANF（100 nM）改变。类似地，ANF诱导的cGMP刺激不受AVP（10 nM）的影响。我们得出结论：1）ANF对培养的皮质集合小管细胞产生cGMP具有直接刺激作用; 2）ANF和AVP之间的任何相互作用都可能发生在环核苷酸形成的远端步骤。
The cellular mechanism of the action of atrial natriuretic factor (ANF) is thought to involve activation of guanylate cyclase. Increasing evidence shows a direct tubular effect of ANF. Part of the ANF-induced diuresis has been suggested to be due to inhibition of the action of arginine vasopressin (AVP) in the cortical collecting tubule. In this study we investigated the effect of ANF on cyclic nucleotide production in primary cultures of cortical collecting tubule cells immunodissected with a monoclonal antibody. ANF caused a dose-dependent stimulation in cyclic guanosine 3',5'-monophosphate (cGMP) production; the half-maximal stimulation was observed at approximately 1 nM of ANF. ANF (0.01-100 nM) had no effect on cyclic adenosine 3',5'-monophosphate (cAMP) accumulation in cortical collecting tubule cultures. AVP caused a dose-dependent increase in cAMP production, and this effect was not altered by the simultaneous addition of ANF (100 nM). Similarly, ANF-induced cGMP stimulation was not influenced by AVP (10 nM). We conclude that 1) ANF has a direct stimulatory action on cGMP production by cultured cortical collecting tubule cells and 2) any interaction between ANF and AVP is likely to occur at steps distal to cyclic nucleotide formation.